A simple tactic for electrochemical determination of a typical biomarker for breast cancer, human epidermal growth factor receptor 2 (HER2), was presented via the construction of a low fouling sensing interface functionalized with polyethylene glycol (PEG) and peptide. The HER2 biosensor was developed based on an electrode modified by the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and Au nanoparticles (AuNPs) as the sensing substrate, and followed by the immobilization of an antifouling PEG and a peptide with both recognizing and antifouling properties. Thanks to the combined antifouling effect of the PEG and peptide, and the specific recognizing ability of the peptide to the target HER2, the developed electrochemical biosensor exhibited strong antifouling performances in complex biofluids, such as human blood and serum, and it was capable of assaying target HER2 within a very wide linear range (1.0 pg·mL⁻¹ to 1.0 μg·mL⁻¹), with an ultralow limit of detection (0.44 pg·mL⁻¹). The combination of two kinds of antifouling biomaterials (PEG and peptide) offered an effective strategy for the development of low fouling sensing platforms suitable for practical assay in complex biotic environments.

通过构建用聚乙二醇 (PEG) 和肽功能化的低污染传感界面，提出了一种用于电化学确定乳腺癌典型生物标志物人表皮生长因子受体 2 (HER2) 的简单策略。HER2 生物传感器是以导电聚合物聚（3,4-乙烯二氧噻吩）（PEDOT）和金纳米粒子（AuNPs）修饰的电极为传感底物，然后固定化防污 PEG 和具有两者的肽而开发的。识别和防污性能。由于PEG和肽的联合防污作用，以及肽对靶点HER2的特异性识别能力，开发的电化学生物传感器在复杂的生物流体（如人体血液和血清）中表现出强大的防污性能，⁻¹至 1.0 μg·mL ⁻¹ )，具有超低检测限 (0.44 pg·mL ⁻¹ )。两种防污生物材料（PEG 和肽）的结合为开发适用于复杂生物环境中实际检测的低污染传感平台提供了有效策略。