Flexible wearable microfluidic devices show great feasibility and potential development in the collection and analysis of sweat due to their convenience and non-invasive characteristics in health-level feedback and disease prediction. However, the traditional production process of microfluidic patches relies on resource-intensive laboratory and high-cost facilities. In this paper, a low-cost laser-cutting technology is proposed to fabricate epidermal microfluidic patches for the collection, storage and colorimetric analysis of sweat. Two different types of capillary bursting valves are designed and integrated into microchannel layers to produce two-stage bursting pressure for the reliable routing of sweat into microreservoirs in sequential fashion, avoiding the mixing of old and new sweat. Additionally, an enzyme-based reagent is embedded into the microreservoirs to quantify the glucose level in sweat by using colorimetric methods, demonstrating a high detection sensitivity at the glucose concentration from 0.1 mM to 1 mM in sweat and an excellent anti-interference performance that prevents interference from substances probably existent in sweat. In vitro and on-body experiments demonstrate the validity of the low-cost, laser-cut epidermal microfluidic patch for the chronological analysis of sweat glucose concentration and its potential application in the monitoring of human physiological information.

中文翻译：

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带有毛细管爆破阀的激光切割表皮微流体贴片，用于按时间顺序捕获、存储和比色传感汗液

柔性可穿戴微流控设备因其在健康水平反馈和疾病预测方面的便利性和非侵入性特点，在汗液收集和分析方面显示出巨大的可行性和发展潜力。然而，微流控贴片的传统生产过程依赖于资源密集型实验室和高成本设施。在本文中，提出了一种低成本的激光切割技术来制造表皮微流体贴片，用于汗液的收集、储存和比色分析。设计了两种不同类型的毛细管爆破阀并将其集成到微通道层中，以产生两级爆破压力，以将汗液可靠地按顺序输送到微储液器中，避免新旧汗液混合。此外，将基于酶的试剂嵌入微库中，通过比色法量化汗液中的葡萄糖水平，在汗液中0.1 mM至1 mM的葡萄糖浓度范围内具有很高的检测灵敏度，并且具有出色的抗干扰性能，可防止干扰汗液中可能存在的物质。体外和体内实验证明了低成本激光切割表皮微流体贴片对汗液葡萄糖浓度的时间顺序分析及其在人体生理信息监测中的潜在应用的有效性。汗液中1mM至1mM，抗干扰性能极佳，可防止汗液中可能存在的物质干扰。体外和体内实验证明了低成本激光切割表皮微流体贴片对汗液葡萄糖浓度的时间顺序分析及其在人体生理信息监测中的潜在应用的有效性。汗液中1mM至1mM，抗干扰性能极佳，可防止汗液中可能存在的物质干扰。体外和体内实验证明了低成本激光切割表皮微流体贴片对汗液葡萄糖浓度的时间顺序分析及其在人体生理信息监测中的潜在应用的有效性。