Characterization of immune cells is essential to advance our understanding of immunology and flow cytometry is an important tool in this context. Addressing both cellular phenotype and antigen-specific functional responses of the same cells is valuable to achieve a more integrated understanding of immune cell behavior and maximizes information obtained from precious samples. Until recently, panel size was limiting, resulting in panels generally focused on either deep immunophenotyping or functional readouts. Ongoing developments in the field of (spectral) flow cytometry have made panels of 30⁺ markers more accessible, opening up possibilities for advanced integrated analyses. Here, we optimized immune phenotyping by co-detection of markers covering chemokine receptors, cytokines and specific T cell/peptide tetramer interaction using a 32-color panel. Such panels enable integrated analysis of cellular phenotypes and markers assessing the quality of immune responses and will contribute to our understanding of the immune system.

中文翻译：

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人 PBMC 的广泛流式细胞术免疫表型分析，包括趋化因子受体、细胞因子和四聚体的检测


免疫细胞的表征对于促进我们对免疫学的理解至关重要，而流式细胞术在这种情况下是一个重要的工具。解决相同细胞的细胞表型和抗原特异性功能反应对于更全面地了解免疫细胞行为并最大限度地利用从珍贵样品中获得的信息很有价值。直到最近，panel的规模还受到限制，导致panel通常专注于深度免疫表型或功能读数。（光谱）流式细胞术领域的持续发展使 30⁺ 标记物的检测组合更容易获得，为高级集成分析开辟了可能性。在这里，我们通过使用 32 色组合共检测涵盖趋化因子受体、细胞因子和特异性 T 细胞/肽四聚体相互作用的标志物来优化免疫表型。此类面板能够对细胞表型和标志物进行综合分析，以评估免疫反应的质量，并将有助于我们对免疫系统的理解。