Microchimica Acta ( IF 5.3 ) Pub Date : 2023-03-08 , DOI: 10.1007/s00604-023-05715-0 Feng He 1, 2 , Tengfei Li 2 , Haijie Wang 1, 3 , Pengfei Du 1 , Weiting Wang 1 , Tianyu Tan 2 , Yaobo Liu 1 , Shoujing Wang 1 , Yanli Ma 1 , Yuanshang Wang 1, 4 , Peng Hu 1 , A M Abd El-Aty 5, 6, 7
|
Screening for persistent organic pollutants (POPs) in food is a complex and challenging process, as POPs can be present in very low levels and can be difficult to detect. Herein, we developed an ultrasensitive biosensor based on a rolling circle amplification (RCA) platform using a glucometer to determine POP. The biosensor was constructed using gold nanoparticle probes modified with antibodies and dozens of primers, magnetic microparticle probes conjugated with haptens, and targets. After competition, RCA reactions are triggered, numerous RCA products hybridize with the ssDNA-invertase, and the target is successfully transformed into glucose. Using ractopamine as a model analyte, this strategy obtained a linear detection range of 0.038–5.00 ng mL−1 and a detection limit of 0.0158 ng mL−1, which was preliminarily verified by screening in real samples. Compared with conventional immunoassays, this biosensor utilizes the high efficiency of RCA and the portable properties of a glucometer, which effectively improves the sensitivity and simplifies the procedures using magnetic separation technology. Moreover, it has been successfully applied to ractopamine determination in animal-derived foods, revealing its potential as a promising tool for POP screening.
Graphical Abstract
中文翻译：
基于血糖仪的生物传感器，用于使用滚环扩增法测定动物源性食品中的莱克多巴胺
筛查食品中的持久性有机污染物 (POP) 是一个复杂且具有挑战性的过程，因为 POP 的含量可能非常低，而且很难检测到。在此，我们开发了一种基于滚环扩增 (RCA) 平台的超灵敏生物传感器，使用血糖仪测定 POP。该生物传感器是使用用抗体和数十种引物修饰的金纳米粒子探针、与半抗原结合的磁性微粒探针和靶标构建的。竞争后触发RCA反应，大量RCA产物与ssDNA-转化酶杂交，靶标成功转化为葡萄糖。使用莱克多巴胺作为模型分析物，该策略获得了 0.038–5.00 ng mL -1的线性检测范围和 0.0158 ng mL -1的检测限, 通过在真实样品中的筛选得到了初步验证。与传统的免疫检测相比，该生物传感器利用了RCA的高效率和血糖仪的便携特性，使用磁分离技术有效提高了灵敏度并简化了程序。此外，它已成功应用于动物源性食品中莱克多巴胺的测定，显示出其作为持久性有机污染物筛查工具的潜力。
京公网安备 11010802027423号