Industrial ethanol production usually requires the costly use of commercial cellulase. Herein, we describe the direct use of cellulase produced by Trichoderma reesei RUT-C30 with sugarcane bagasse (SCB) as a substrate for the production of ethanol. The pretreated SCB supplemented with wheat bran (weight ratio of 4:1) was used for T. reesei cellulase production by solid-state fermentation. The cellulase activity titers of 5.5 ± 0.7 FPU/gdm was achieved. The activity increased to 15.0 ± 0.9 FPU/gdm when lactose was used as an inducer. The cellulase contained SCB (CSCB) was used directly as the raw material for ethanol production by Kluyveromyces marxianus at 40 °C, yielding 14.1 ± 1.8% (w/w) of ethanol. Supplementing 5 FPU/gdm of commercial cellulase (Cellic® CTec 2) to CSCB improved the ethanol yield to 30.3 ± 0.4% (w/w). Supplementing Cellic® CTec 2 provided large amount of β-glucosidase activity to CSCB, which hydrolyzes cellobiose to glucose, thereby preventing the inhibition of cellobiose to exoglucanase and endoglucanase. This study demonstrate the feasibility of integrating direct utilization of CSCB without further processing, conversion of CSCB to fermentable sugars, and fermentation of yeast to ethanol in a single reactor. This may be the most efficient way to convert biomass to valuable ethanol.

工业乙醇生产通常需要使用昂贵的商业纤维素酶。在此，我们描述了直接使用由里氏木霉RUT-C30 产生的纤维素酶和甘蔗渣 (SCB) 作为生产乙醇的底物。预处理的 SCB 补充有麦麸（重量比为 4:1）用于通过固态发酵生产里氏木霉纤维素酶。实现了 5.5 ± 0.7 FPU/gdm 的纤维素酶活性滴度。当乳糖用作诱导剂时，活性增加到 15.0 ± 0.9 FPU/gdm。含纤维素酶的SCB(CSCB)直接作为马克斯克鲁维酵母生产乙醇的原料在 40 °C 下，产生 14.1 ± 1.8%（w/w）的乙醇。向 CSCB 添加 5 FPU/gdm 的商业纤维素酶 (Cellic® CTec 2) 可将乙醇产量提高至 30.3 ± 0.4% (w/w)。补充 Cellic® CTec 2 为 CSCB 提供了大量的 β-葡萄糖苷酶活性，将纤维二糖水解为葡萄糖，从而防止纤维二糖对外切葡聚糖酶和内切葡聚糖酶的抑制。本研究证明了在单个反应器中整合直接利用 CSCB 而不进行进一步加工、将 CSCB 转化为可发酵糖以及将酵母发酵为乙醇的可行性。这可能是将生物质转化为有价值的乙醇的最有效方式。