Fangwen Jiuwei Decoction is a traditional Chinese medicine preparation for the treatment of pneumonia developed by Shenzhen Bao'an Chinese Medicine Hospital, which shows remarkable clinical responses. Qualitative and quantitative analyses of the main active compounds are crucial for the quality control of traditional Chinese medicine prescription in clinical application. In this study, we identified nine active compounds essential for the pharmacological effects of Fangwen Jiuwei Decoction based on the analysis of the Network Pharmacology and relevant literature. Moreover, these compounds can interact with several crucial drug targets in pneumonia based on molecular docking. We applied high-performance liquid chromatography–tandem mass spectrometry method was established these nine active ingredients’ qualitative and quantitative detections. The possible cleavage pathways of nine active components were determined based on secondary ions mass spectrometry. The results of high-performance liquid chromatography–tandem mass spectrometry were further validated, which show a satisfactory correlation coefficient (r > 0.99), recovery rate (≥93.31%), repeatability rate (≤5.62%), stability (≤7.95%), intra-day precision (≤6.68%), and inter-day precision (≤9.78%). The limit of detection was as low as 0.01 ng/ml. In this study, we established a high-performance liquid chromatography–tandem mass spectrometry method to qualitatively and quantitatively analyze the chemical components in the Fangwen Jiuwei Decoction extract.

中文翻译：

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液相色谱-质谱法对防瘟九味汤中九种化合物的鉴定与定量

防瘟九味汤是深圳市宝安中医院研制开发的治疗肺炎的中药制剂，临床疗效显着。主要活性成分的定性和定量分析对于临床应用中药方剂的质量控制至关重要。在这项研究中，我们基于网络药理学和相关文献的分析，确定了九种对防瘟九胃汤药理作用必不可少的活性成分。此外，基于分子对接，这些化合物可以与肺炎中的几个关键药物靶点相互作用。我们应用高效液相色谱-串联质谱法建立了这九种有效成分的定性和定量检测。基于二次离子质谱法确定了九种活性成分可能的裂解途径。高效液相色谱-串联质谱的结果得到进一步验证，显示出令人满意的相关系数（r  > 0.99）、回收率（≥93.31%）、重复性（≤5.62%）、稳定性（≤7.95%）、日内精密度（≤6.68%）、日间精密度（≤9.78%）。检测限低至 0.01 ng/ml。在本研究中，我们建立了高效液相色谱-串联质谱法对防瘟九味汤提取物中的化学成分进行定性和定量分析。