HSP90.2 promotes CO2 assimilation rate, grain weight and yield in wheat,Plant Biotechnology Journal

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HSP90.2 promotes CO2 assimilation rate, grain weight and yield in wheat
Plant Biotechnology Journal ( IF 10.1 ) Pub Date : 2023-02-16 , DOI: 10.1111/pbi.14032
Yan Yan _{1,

2} , Meng-Lu Wang ₁ , Yue-Ting Guo _{1,

3} , Ci-Hang Ding _{1,

3} , Ke-Xin Niu _{1,

3} , Xiao-Ming Li _{1,

3} , Congwei Sun ₂ , Zhongdong Dong ₂ , Dangqun Cui ₂ , Awais Rasheed ₄ , Chenyang Hao ₅ , Xueyong Zhang ₅ , Ganggang Guo ₆ , Zhongfu Ni ₁ , Qixin Sun ₁ , Feng Chen ₂ , Jin-Ying Gou 缑金营 _{1,

3}

Affiliation

Wheat fixes CO₂ by photosynthesis into kernels to nourish humankind. Improving the photosynthesis rate is a major driving force in assimilating atmospheric CO₂ and guaranteeing food supply for human beings. Strategies for achieving the above goal need to be improved. Here, we report the cloning and mechanism of CO₂ ASSIMILATION RATE AND KERNEL-ENHANCED 1 (CAKE1) from durum wheat (Triticum turgidum L. var. durum). The cake1 mutant displayed a lower photosynthesis rate with smaller grains. Genetic studies identified CAKE1 as HSP90.2-B, encoding cytosolic molecular chaperone folding nascent preproteins. The disturbance of HSP90.2 decreased leaf photosynthesis rate, kernel weight (KW) and yield. Nevertheless, HSP90.2 over-expression increased KW. HSP90.2 recruited and was essential for the chloroplast localization of nuclear-encoded photosynthesis units, for example PsbO. Actin microfilaments docked on the chloroplast surface interacted with HSP90.2 as a subcellular track towards chloroplasts. A natural variation in the hexaploid wheat HSP90.2-B promoter increased its transcription activity, enhanced photosynthesis rate and improved KW and yield. Our study illustrated an HSP90.2–Actin complex sorting client preproteins towards chloroplasts to promote CO₂ assimilation and crop production. The beneficial haplotype of Hsp90.2 is rare in modern varieties and could be an excellent molecular switch promoting photosynthesis rate to increase yield in future elite wheat varieties.

中文翻译：

HSP90.2 促进小麦 CO2 同化率、粒重和产量

小麦通过光合作用将 CO ₂固定到籽粒中以滋养人类。_{提高光合作用速率是吸收大气中CO 2}和保证人类食物供应的主要动力。实现上述目标的策略需要改进。在这里，我们报道了来自硬粒小麦（Triticum turgidum L. var. durum ）的CO ₂ 同化率和KERNEL-ENHANCED 1（CAKE1 ）的克隆和机制。cake1突变体显示出较低的光合速率和较小的颗粒。遗传学研究将CAKE1鉴定为HSP90.2-B, 编码胞质分子伴侣折叠新生前蛋白。HSP90.2的干扰降低了叶片光合速率、粒重（KW）和产量。然而，HSP90.2过表达增加了 KW。HSP90.2 招募并且对于核编码光合作用单位（例如 PsbO）的叶绿体定位至关重要。停靠在叶绿体表面的肌动蛋白微丝与 HSP90.2 相互作用，作为叶绿体的亚细胞轨道。六倍体小麦HSP90.2-B启动子的自然变异增加了其转录活性，提高了光合速率并提高了千瓦和产量。我们的研究说明了 HSP90.2-肌动蛋白复合物将客户前体蛋白分选到叶绿体以促进 CO ₂同化和作物生产。Hsp90.2的有益单倍型在现代品种中很少见，可能是未来优质小麦品种提高光合速率提高产量的优良分子开关。

更新日期：2023-02-16

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