This study focused on a glazing layer made by modified bio-polymer to inhibit chemical variations in super-chilled postmortem fish. Essential oils were extracted from tangerine peel and their major antioxidant and antimicrobial components were identified. The essential oils were introduced into chitosan material as functional agents to improve the performance as freshness-keeping glazing layers for super-chilled tilapia. During the storage, antimicrobial effects of glazing significantly decreased the bacterial growth, as the growth of total bacterial count (TBC) decreased from ∼12.8 log CFU/g to ∼ 3.5 log CFU/g and that of total aerobic count (TAC) decreased from ∼11.1 log CFU/g to ∼ 3.3 log CFU/g. Antioxidant effects of glazing led to decreases of peroxide value (PV) from 6.41 meq/kg to ∼2.5 meq/kg and thiobarbituric acid reactive substances (TBARS) content from 1.09 mg MDA/kg to ∼ 0.5 mg MDA/kg at the end of the storage. Glazing inhibited the change of corruption degree reflected by K-value and protein decomposition reflected by the contents of trichloroacetic acid (TCA) soluble peptides and total volatile basic nitrogen (TVB-N). Glazing also limited the contents of main biogenic amines (histamine, putrescine, cadaverine, spermine, spermidine, tyramine, tryptamine) within relatively low levels.

本研究重点关注由改性生物聚合物制成的玻璃层，以抑制超冷死后鱼的化学变化。从陈皮中提取精油，并确定了它们的主要抗氧化和抗菌成分。将精油作为功能剂引入壳聚糖材料中，以提高超冷罗非鱼保鲜上光层的性能。在储存期间，玻璃的抗菌作用显着降低了细菌的生长，因为总细菌数 (TBC) 的增长从～12.8 log CFU/g 下降到～3.5 log CFU/g，总需氧菌数 (TAC) 从∼11.1 log CFU/g 至∼ 3.3 log CFU/g。玻璃的抗氧化作用导致过氧化值 (PV) 从 6.41 meq/kg 降至 ~2。5 meq/kg 和硫代巴比妥酸反应物质 (TBARS) 含量从 1.09 mg MDA/kg 到储存结束时的 ∼ 0.5 mg MDA/kg。施釉抑制了K值反映的腐败程度变化和三氯乙酸（TCA）可溶性肽和总挥发性碱氮（TVB-N）含量反映的蛋白质分解。施釉还将主要生物胺（组胺、腐胺、尸胺、精胺、亚精胺、酪胺、色胺）的含量限制在相对较低的水平。