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Impedimetric Nanobiosensor for the Detection of SARS-CoV-2 Antigens and Antibodies
ACS Sensors ( IF 8.2 ) Pub Date : 2023-02-10 , DOI: 10.1021/acssensors.2c01686 Diana Isabel Sandoval Bojórquez 1 , Željko Janićijević 1 , Brenda Palestina Romero 1 , Eduardo Sergio Oliveros Mata 2 , Markus Laube 1 , Anja Feldmann 1 , Alexandra Kegler 1 , Laura Drewitz 1 , Ciarán Fowley 2 , Jens Pietzsch 1, 3 , Juergen Fassbender 2 , Torsten Tonn 4, 5 , Michael Bachmann 1, 6, 7, 8, 9 , Larysa Baraban 1
ACS Sensors ( IF 8.2 ) Pub Date : 2023-02-10 , DOI: 10.1021/acssensors.2c01686 Diana Isabel Sandoval Bojórquez 1 , Željko Janićijević 1 , Brenda Palestina Romero 1 , Eduardo Sergio Oliveros Mata 2 , Markus Laube 1 , Anja Feldmann 1 , Alexandra Kegler 1 , Laura Drewitz 1 , Ciarán Fowley 2 , Jens Pietzsch 1, 3 , Juergen Fassbender 2 , Torsten Tonn 4, 5 , Michael Bachmann 1, 6, 7, 8, 9 , Larysa Baraban 1
Affiliation
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Detection of antigens and antibodies (Abs) is of great importance in determining the infection and immunity status of the population, as they are key parameters guiding the handling of pandemics. Current point-of-care (POC) devices are a convenient option for rapid screening; however, their sensitivity requires further improvement. We present an interdigitated gold nanowire-based impedance nanobiosensor to detect COVID-19-associated antigens (receptor-binding domain of S1 protein of the SARS-CoV-2 virus) and respective Abs appearing during and after infection. The electrochemical impedance spectroscopy technique was used to assess the changes in measured impedance resulting from the binding of respective analytes to the surface of the chip. After 20 min of incubation, the sensor devices demonstrate a high sensitivity of about 57 pS·sn per concentration decade and a limit of detection (LOD) of 0.99 pg/mL for anti-SARS-CoV-2 Abs and a sensitivity of around 21 pS·sn per concentration decade and an LOD of 0.14 pg/mL for the virus antigen detection. Finally, the analysis of clinical plasma samples demonstrates the applicability of the developed platform to assist clinicians and authorities in determining the infection or immunity status of the patients.
中文翻译：
用于检测 SARS-CoV-2 抗原和抗体的阻抗纳米生物传感器
抗原和抗体（Ab）的检测对于确定人群的感染和免疫状态非常重要，因为它们是指导应对流行病的关键参数。目前的护理点 (POC) 设备是快速筛查的便捷选择；然而，它们的灵敏度需要进一步提高。我们提出了一种基于叉指金纳米线的阻抗纳米生物传感器，用于检测 COVID-19 相关抗原（SARS-CoV-2 病毒 S1 蛋白的受体结合域）以及感染期间和感染后出现的相应抗体。电化学阻抗谱技术用于评估由于各分析物与芯片表面的结合而引起的测量阻抗的变化。孵育 20 分钟后，传感器装置表现出每浓度十年约 57 pS·s n的高灵敏度，抗 SARS-CoV-2 抗体的检测限 (LOD) 为 0.99 pg/mL，灵敏度约为病毒抗原检测的每浓度十年为21 pS·s n ，LOD 为 0.14 pg/mL。最后，对临床血浆样本的分析证明了所开发平台的适用性，可帮助临床医生和当局确定患者的感染或免疫状态。
更新日期：2023-02-10
中文翻译：
用于检测 SARS-CoV-2 抗原和抗体的阻抗纳米生物传感器
抗原和抗体（Ab）的检测对于确定人群的感染和免疫状态非常重要，因为它们是指导应对流行病的关键参数。目前的护理点 (POC) 设备是快速筛查的便捷选择；然而，它们的灵敏度需要进一步提高。我们提出了一种基于叉指金纳米线的阻抗纳米生物传感器，用于检测 COVID-19 相关抗原（SARS-CoV-2 病毒 S1 蛋白的受体结合域）以及感染期间和感染后出现的相应抗体。电化学阻抗谱技术用于评估由于各分析物与芯片表面的结合而引起的测量阻抗的变化。孵育 20 分钟后，传感器装置表现出每浓度十年约 57 pS·s n的高灵敏度，抗 SARS-CoV-2 抗体的检测限 (LOD) 为 0.99 pg/mL，灵敏度约为病毒抗原检测的每浓度十年为21 pS·s n ，LOD 为 0.14 pg/mL。最后，对临床血浆样本的分析证明了所开发平台的适用性，可帮助临床医生和当局确定患者的感染或免疫状态。
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