For the diagnosis and therapy of small cell lung cancer (SCLC), the accurate and sensitive determination of neuron-specific enolase (NSE) content is crucial. This work outlines a dual-quenching electrochemiluminescence resonance energy transfer (ECL-RET) immunosensor based on the double quenching effects of iron base metal organic frameworks (FeMOFs) loaded with small sized CuO nanoparticles (FeMOFs-sCuO) towards CoPd nanoparticles (CoPdNPs) enhanced porous g-C₃N₄ (P–C₃N₄-CoPdNPs). To be specific, we prepared a porous g-C₃N₄ (P–C₃N₄) which has a rich porous structure, and significantly increased the specific surface area and the number of reaction sites of P–C₃N₄. Meanwhile, the CoPdNPs were loaded onto P–C₃N₄ to improve the ECL luminescence property of P–C₃N₄/K₂S₂O₈ system through acting as a coreaction accelerator. In addition, the ultraviolet–visible (UV–vis) absorption spectra of FeMOFs and small sized CuO nanoparticles (sCuO) showed considerable overlap with the ECL emission spectra of P–C₃N₄ appropriately. Therefore, FeMOFs with high specific surface area were prepared and well combined with sCuO to effectively dual-quenching the ECL emission of P–C₃N₄ based on resonance energy transfer. Hence, a new type ECL-RET couple made up of P–C₃N₄-CoPdNPs (donor) and FeMOFs-sCuO (acceptor) were developed for the first time. A certain amount of P–C₃N₄-CoPdNPs, Ab₁, BSA, NSE were modified layer by layer onto the electrode surface. Then FeMOFs-sCuO-Ab₂ bioconjugates was incubated through the immune recognition binding. As a result, a sandwich-type ECL biosensor was manufactured successfully for NSE immunoassay. Under optimal experimental conditions, the limit of detection (LOD) and the limit of quantitation (LOQ) of the prepared ECL sensor for NSE analysis was 20.4 fg mL⁻¹ and 7.99 fg mL⁻¹, respectively, with the relative standard deviation (RSD) of 1.68%. The linear detection range was 0.0000500–100 ng mL⁻¹. The studied immunosensor had satisfactory sensitivity, specificity and reproducibility, manifesting the suggested sensing strategy might offer a good technical means and theoretical basis for the sensitivity analysis of NSE and has a potential application in clinical diagnosis analysis.

对于小细胞肺癌 (SCLC) 的诊断和治疗，准确灵敏地测定神经元特异性烯醇化酶 (NSE) 含量至关重要。这项工作概述了一种双猝灭电化学发光共振能量转移 (ECL-RET) 免疫传感器，它基于负载有小尺寸 CuO 纳米粒子 (FeMOFs-sCuO) 的铁基金属有机框架 (FeMOFs) 对 CoPd 纳米粒子 (CoPdNPs) 增强的双猝灭效应多孔 gC ₃ N ₄ (P–C ₃ N ₄ -CoPdNPs)。具体而言，我们制备了多孔 gC ₃ N ₄ (P–C ₃ N _{4)具有丰富的多孔结构，显着提高了P-C 3} N ₄的比表面积和反应位点数量。同时，将CoPdNPs负载到P-C ₃ N ₄上，通过作为共反应促进剂提高P-C ₃ N ₄ /K ₂ S ₂ O _{8体系的ECL发光性能。}此外，FeMOF 和小尺寸 CuO 纳米粒子 (sCuO) 的紫外-可见 (UV-vis) 吸收光谱与 P-C 3 N 4 的 ECL 发射光谱有相当_大的_重叠适当地。因此，制备了具有高比表面积的 FeMOFs 并与 sCuO 良好结合，基于共振能量转移有效地双猝灭 P–C ₃ N _{4的 ECL 发射。}因此，首次开发了由P–C ₃ N ₄ -CoPdNPs（供体）和FeMOFs-sCuO（受体）组成的新型ECL-RET对。在电极表面逐层修饰一定量的P–C ₃ N ₄ -CoPdNPs、Ab _{1 、BSA、NSE。}然后是FeMOFs-sCuO-Ab ₂通过免疫识别结合孵育生物偶联物。结果，成功制造了用于 NSE 免疫测定的夹心型 ECL 生物传感器。在最佳实验条件下，制备的用于NSE分析的ECL传感器的检测限（LOD）和定量限（LOQ）分别为20.4 fg mL -1 和7.99 fg ^mL -1 ^，相对标准偏差（RSD ) 的 1.68%。线性检测范围为 0.0000500–100 ng mL ^-1。所研究的免疫传感器具有令人满意的灵敏度、特异性和重现性，表明所提出的传感策略可为NSE的灵敏度分析提供良好的技术手段和理论基础，在临床诊断分析中具有潜在的应用价值。