Mobile and Self-Sustained Data Storage in an Extremophile Genomic DNA,Advanced Science

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Mobile and Self-Sustained Data Storage in an Extremophile Genomic DNA
Advanced Science ( IF 14.3 ) Pub Date : 2023-02-03 , DOI: 10.1002/advs.202206201
Fajia Sun ₁ , Yiming Dong ₁ , Ming Ni ₂ , Zhi Ping ₂ , Yuhui Sun ₂ , Qi Ouyang _{1,

3} , Long Qian ₁

Affiliation

DNA has been pursued as a novel biomaterial for digital data storage. While large-scale data storage and random access have been achieved in DNA oligonucleotide pools, repeated data accessing requires constant data replenishment, and these implementations are confined in professional facilities. Here, a mobile data storage system in the genome of the extremophile Halomonas bluephagenesis, which enables dual-mode storage, dynamic data maintenance, rapid readout, and robust recovery. The system relies on two key components: A versatile genetic toolbox for the integration of 10–100 kb scale synthetic DNA into H. bluephagenesis genome and an efficient error correction coding scheme targeting noisy nanopore sequencing reads. The storage and repeated retrieval of 5 KB data under non-laboratory conditions are demonstrated. The work highlights the potential of DNA data storage in domestic and field scenarios, and expands its application domain from archival data to frequently accessed data.

中文翻译：

极端微生物基因组 DNA 中的移动和自我维持数据存储

DNA 一直被视为用于数字数据存储的新型生物材料。虽然在 DNA 寡核苷酸池中已经实现了大规模数据存储和随机访问，但重复数据访问需要不断补充数据，而这些实现仅限于专业设施。此处是极端微生物Halomonas bluephagenesis基因组中的移动数据存储系统，可实现双模式存储、动态数据维护、快速读出和稳健恢复。该系统依赖于两个关键组件：一个多功能的遗传工具箱，用于将 10-100 kb 规模的合成 DNA 整合到H. bluephagenesis中基因组和针对噪声纳米孔测序读数的有效纠错编码方案。演示了非实验室条件下 5 KB 数据的存储和重复检索。该工作突出了 DNA 数据存储在国内和野外场景中的潜力，并将其应用领域从档案数据扩展到经常访问的数据。

更新日期：2023-02-03

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