Due to increasing industrialization and overpopulation, the amount of toxic metals is increasing in the environment, including air, soil, water, and food. Solid phase extraction is an efficient and ideal technique to preconcentrate the toxic metals before their measurements by analytical instruments. Russula brevipes was immobilized on γ-Fe₂O₃ magnetic nanoparticles and employed as a SPE sorbent to preconcentrate the trace level of Pb(II), Mn(II), and Co(II). To investigate the extraction conditions, significant experimental parameters were examined in details. LODs were calculated as 0.022, 0.015, and 0.024 ng mL⁻¹ for Pb(II), Mn(II), and Co(II), respectively. The biosorption capacities of R. brevipes immobilized γ-Fe₂O₃ were calculated as 43.1 mg g⁻¹ for Pb(II), 54.9 mg g⁻¹ for Mn(II), and 49.7 mg g⁻¹ for Co(II). Pb(II), Mn(II), and Co(II) in food samples at trace levels were preconcentrated by applying the developed method.

由于工业化进程加快和人口过剩，环境中的有毒金属含量不断增加，包括空气、土壤、水和食物。固相萃取是一种有效且理想的技术，可在分析仪器测量有毒金属之前对其进行预浓缩。将Russula brevipes固定在 γ-Fe ₂ O ₃磁性纳米颗粒上并用作 SPE 吸附剂以预浓缩痕量水平的 Pb(II)、Mn(II) 和 Co(II)。为了研究提取条件，详细检查了重要的实验参数。Pb(II)、Mn(II) 和 Co(II)的 LOD 计算值分别为 0.022、0.015 和 0.024 ng mL ^-1。R. brevipes的生物吸附能力固定化的 γ-Fe ₂ O ₃计算为Pb(II)为 43.1 mg g ^{-1 ，Mn(II) 为 54.9 mg g -1}，Co(II) 为49.7 mg g ^{-1 。}通过应用开发的方法预浓缩食品样品中痕量的 Pb(II)、Mn(II) 和 Co(II)。