Anthocyanins are bioactive compounds found in blueberries. However, their poor bioavailability restricts their functional activities in vivo, which is a challenging issue in the application of blueberry anthocyanins. Our current study utilized α-casein as a carrier and analyzed its influence on the excretion of blueberry anthocyanins in urine and feces in a rat model to reflect the enhanced bioavailability of blueberry anthocyanins by α-casein in vivo. The results showed that α-casein suppressed the excretive content of blueberry anthocyanins (malvidin-3-O-galacoside (M3G), cyanidin-3-O-glucoside (C3G), and delphinidin-3-O-glucoside (D3G)), increased the content of metabolites in urine (syringic acid, ferulic acid, 4-hydroxybenzoic acid, and vanillic acid), and reduced metabolite content in feces (syringic acid, ferulic acid, and gallic acid), indicating that α-casein was effective in controlling the excretion of blueberry anthocyanins and their metabolites. In summary, these results provided sufficient evidence for the positive effects of α-casein on the bioavailability of blueberry anthocyanins.

花青素是在蓝莓中发现的生物活性化合物。然而，它们较差的生物利用度限制了它们在体内的功能活性，这在蓝莓花青素的应用中是一个具有挑战性的问题。我们目前的研究以α-酪蛋白为载体，通过大鼠模型分析其对尿液和粪便中蓝莓花青素排泄的影响，以反映α-酪蛋白在体内增强蓝莓花青素的生物利用度。结果表明，α-酪蛋白抑制了蓝莓花青素（malvidin-3- O -galacoside (M3G)、cyanidin-3- O -glucoside (C3G) 和 delphinidin-3- O-葡萄糖苷（D3G）），尿液中代谢物（丁香酸、阿魏酸、4-羟基苯甲酸、香草酸）含量增加，粪便中代谢物（丁香酸、阿魏酸、没食子酸）含量降低，表明α-酪蛋白可有效控制蓝莓花青素及其代谢产物的排泄。总之，这些结果为α-酪蛋白对蓝莓花青素生物利用度的积极影响提供了充分的证据。