Food Chemistry ( IF 8.8 ) Pub Date : 2023-01-24 , DOI: 10.1016/j.foodchem.2023.135562 Quanshou Feng 1 , Zehu Xie 1 , Hongze Liang 1 , Chuan-Fan Ding 1 , Yinghua Yan 1
Ovalbumin (OVA) is an important protein source in our daily life. Unfortunately, the food safety problem has become more and more serious, such as protein allergy and contaminated protein. Therefore, it is necessary to detect vital proteins efficiently and rapidly. Mass spectrometry (MS) is a powerful tool for the detection of proteins. Herein, dual amino acids functionalized covalent organic frameworks containing disulfide covalent bonds (COF@SS@GC, where G is glutathione and C is cysteine) were facilely prepared for OVA enrichment through hydrophilic interaction liquid chromatography (HILIC) under physiological pH. The results showed that COF@SS@GC had displayed sensitive detection (0.1 fmol), good selectivity (OVA: BSA = 1:100), adsorption capacity (311 mg/g), stability, reproducibility, linearity, LOQ level (42 μg/mL) and recovery ratio (64.83 %) for OVA. COF@SS@GC also demonstrated satisfactory purification ability in the enrichment of egg white, indicating that COF@SS@GC had great potential in the enrichment of protein from complex samples.
中文翻译:
共价有机框架的合成后作为亲水平台,用于在生理 pH 下特异性检测卵清蛋白
卵清蛋白 (OVA) 是我们日常生活中重要的蛋白质来源。不幸的是,食品安全问题越来越严重,例如蛋白质过敏和受污染的蛋白质。因此,有必要高效、快速地检测重要蛋白质。质谱法 (MS) 是检测蛋白质的有力工具。在此,通过亲水相互作用液相色谱 (HILIC) 在生理 pH 条件下轻松制备含有二硫共价键的双氨基酸功能化共价有机骨架(COF@S S @GC,其中 G 是谷胱甘肽,C 是半胱氨酸)用于 OVA 富集。结果表明,COF@SS@GC 表现出灵敏的检测(0.1 fmol)、良好的选择性(OVA: BSA = 1:100)、吸附容量(311 mg/g)、稳定性、重现性、线性、LOQ 水平(42 μg/mL)和回收率OVA (64.83 %)。COF@S S@GC在蛋清富集方面也表现出令人满意的纯化能力,表明COF@S S@GC在复杂样品中富集蛋白质方面具有巨大潜力。