T-butanol is widely used in three-phase partitioning (TPP), which is harmful to the environment. pH-switchable deep eutectic solvents (DESs) can be used as recyclable alternatives to t-butanol. This study aimed to construct DES-based TPP for extracting and purifying grape seed polysaccharides (GSP). The main influence factors were investigated in single-factor experiments. DES-1 (dodecanoic acid: octanoic acid = 1:1)-based extraction was screened, and the extraction yield reached the maximum of 98.04 mg/g under the optimal conditions. Furthermore, DES can be recycled, only suffering a small loss capacity in extraction yield after 25 cycles. Most importantly, the extractability of DES could be completely recovered after switching and regeneration. The molecular weight of obtained GSP was 60 kDa, and the main monosaccharides of GSP included mannose, glucose, galactose, and arabinose. This study provides an efficient and sustainable method for the extraction of bioactive substances.

叔丁醇广泛用于三相分配（TPP），对环境有害。pH 可切换的低共熔溶剂 (DES) 可用作t的可回收替代品-丁醇。本研究旨在构建基于 DES 的 TPP，用于提取和纯化葡萄籽多糖 (GSP)。主要影响因素采用单因素试验进行考察。筛选基于DES-1（十二烷酸：辛酸=1:1）的提取物，在最佳条件下提取得率最高达到98.04 mg/g。此外，DES 可以循环使用，在 25 个循环后提取产量的容量损失很小。最重要的是，DES 的可萃取性在转换和再生后可以完全恢复。所得GSP的分子量为60 kDa，GSP的主要单糖包括甘露糖、葡萄糖、半乳糖和阿拉伯糖。本研究为生物活性物质的提取提供了一种高效且可持续的方法。