In this study, we report ultrafine-sized antibiotic dot particles targeting gram-negative bacteria. The water-soluble ultrafine-sized hyaluronate dot (dHA) was conjugated with polymyxin B (PMB, as a target material for lipopolysaccharide on a gram-negative bacteria) and chlorine e6 (Ce6, as a model photosensitizer), and it was denoted as dHA-(PMB/Ce6). These dot particles interacted with lipopolysaccharide (LPS) on gram-negative bacteria, which enabled PMB-mediated docking to the outer membrane of gram-negative cells. In the antimicrobial experiments using Escherichia coli cells, the phototoxic singlet oxygen generated from the dHA-(PMB/Ce6) under light irradiation inhibited E. coli cell growth. This demonstrated that dHA-(PMB/Ce6) can provide light-activated selective phototoxicity against gram-negative bacteria. We expect that the dot particle system can be used as an efficient method for improved antimicrobial treatment.

中文翻译：

---

用于抗菌光动力疗法的多粘菌素 B/氯 e6 共轭透明质酸点颗粒

在这项研究中，我们报告了针对革兰氏阴性菌的超细抗生素点颗粒。水溶性超细透明质酸点（dHA）与多粘菌素 B（PMB，作为革兰氏阴性菌脂多糖的靶标物质）和氯 e6（Ce6，作为模型光敏剂）结合，表示为dHA-(PMB/Ce6)。这些点状颗粒与革兰氏阴性菌上的脂多糖 (LPS) 相互作用，从而使 PMB 介导的对接至革兰氏阴性细胞的外膜。在使用大肠杆菌细胞的抗菌实验中，光照射下 dHA-(PMB/Ce6) 产生的光毒性单线态氧抑制大肠杆菌细胞生长。这表明 dHA-(PMB/Ce6) 可以提供针对革兰氏阴性菌的光激活选择性光毒性。我们期望点粒子系统可以用作改进抗菌处理的有效方法。