Consumers around the world prefer high quality table grapes. To achieve higher quality traits at ripening, grapevine producers apply different plant growth regulators. The synthetic cytokinin forchlorfenuron N–(2–chloro–4–pyridinyl)–N′–phenylurea (CPPU) is widely used, its effect on grape quality is poorly understood. We hypothesized that the use of CPPU in pre-flowering can lead to changes in the metabolism that affects grape quality at harvest. Therefore, we investigated the role of CPPU applications on the quality of grapes by integrating proteomics and metabolomics. CPPU-treated grapevines showed a significant increase in berry size and firmness. Proteomic analyses indicated that CPPU-treated berries accumulated enzymes associated with carbohydrate metabolism, glycolysis, and tricarboxylic acid (TCA) cycle at harvest. Metabolomic analyses showed shifts in the abundance of compounds associated with carbohydrate metabolism and TCA cycle in CPPU-treated grapes. These findings suggest that CPPU applications modulate central carbon metabolism, improving grape berry quality.

世界各地的消费者都喜欢高品质的鲜食葡萄。为了在成熟时获得更高品质的特性，葡萄种植者使用不同的植物生长调节剂。氯芬脲 N-(2-氯-4-吡啶基)-N'-苯基脲 (CPPU) 的合成细胞分裂素被广泛使用，其对葡萄品质的影响知之甚少。我们假设在开花前使用 CPPU 会导致新陈代谢发生变化，从而影响收获时的葡萄品质。因此，我们通过整合蛋白质组学和代谢组学研究了 CPPU 应用对葡萄品质的作用。经 CPPU 处理的葡萄藤显示出浆果大小和硬度的显着增加。蛋白质组学分析表明，CPPU 处理的浆果在收获时积累了与碳水化合物代谢、糖酵解和三羧酸 (TCA) 循环相关的酶。代谢组学分析显示，在 CPPU 处理的葡萄中，与碳水化合物代谢和 TCA 循环相关的化合物丰度发生了变化。这些发现表明，CPPU 的应用可以调节中心碳代谢，从而提高葡萄果实的品质。