One of the significant limitations of aquaculture worldwide is the prevalence of divalent copper (Cu). Crayfish (Procambarus clarkii) are economically important freshwater species adapted to a variety of environmental stimuli, including heavy metal stresses; however, large-scale transcriptomic data of the hepatopancreas of crayfish in response to Cu stress are still scarce. Here, integrated comparative transcriptome and weighted gene co-expression network analyses were initially applied to investigate gene expression profiles of the hepatopancreas of crayfish subjected to Cu stress for different periods. As a result, 4662 significant differentially expressed genes (DEGs) were identified following Cu stress. Bioinformatics analyses revealed that the "focal adhesion" pathway was one of the most significantly upregulated response pathways following Cu stress, and seven DEGs mapped to this pathway were identified as hub genes. Furthermore, the seven hub genes were examined by quantitative PCR, and each was found to have a substantial increase in transcript abundance, suggesting a critical role of the "focal adhesion" pathway in the response of crayfish to Cu stress. Our transcriptomic data can be a good resource for the functional transcriptomics of crayfish, and these results may provide valuable insights into the molecular response mechanisms underlying crayfish to Cu stress.

全世界水产养殖的重大限制之一是二价铜 (Cu) 的普遍存在。小龙虾（克氏原螯虾) 是经济上重要的淡水物种，适应各种环境刺激，包括重金属压力；然而，小龙虾肝胰腺响应铜胁迫的大规模转录组学数据仍然很少。在这里，整合的比较转录组和加权基因共表达网络分析最初被用于研究不同时期铜胁迫下小龙虾肝胰腺的基因表达谱。结果，在 Cu 胁迫后鉴定了 4662 个显着差异表达基因 (DEG)。生物信息学分析表明，“粘着斑”通路是铜胁迫后最显着上调的反应通路之一，映射到该通路的七个 DEGs 被确定为中枢基因。此外，通过定量 PCR 检查了七个中枢基因，发现每个基因的转录本丰度都有显着增加，这表明“粘着斑”途径在小龙虾对铜胁迫的反应中起着关键作用。我们的转录组学数据可以成为小龙虾功能转录组学的良好资源，这些结果可能为了解小龙虾对铜胁迫的分子反应机制提供有价值的见解。