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High-Performance Electrochemiluminescence of a Coordination-Driven J-Aggregate K-PTC MOF Regulated by Metal–Phenolic Nanoparticles for Biomarker Analysis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-12-19 , DOI: 10.1021/acs.analchem.2c04159
Jinglong Fang 1 , Li Dai 1 , Ruiqing Feng 1 , Dan Wu 1 , Xiang Ren 1 , Wei Cao 1 , Hongmin Ma 1 , Qin Wei 1
Affiliation  

The elimination of aggregation-caused quenching of polycyclic aromatic hydrocarbons by metal–ligand coordination is of immense scientific interest in solid-state electrochemiluminescence (ECL) sensing. Herein potassium ion (K+)-mediated J-aggregate K-PTC MOF (PTCA, perylene-3,4,9,10-tetracarboxylic) was synthesized and employed to formulate an ECL immunosensor for biomarker detection. The coordination-driven aggregates are arranged in an end-to-end side mode, which overcomes the aggregation-caused quenching related to PTCA concentration. Compared with PTCA, K-PTC MOF shows a more stable ECL emission with an unprecedented red shift to 718 nm and is equipped with ECL activity for analytical applications at a voltage of −1.1 V. Considering the requirements of accurate detection, metal–phenolic bioactive nanoparticles (MPNPs) were synthesized for the construction of a sandwich sensing platform to realize the steady-state regulation of ECL. As proof of applicability, a constructive experiment was carried out with neuron-specific enolase (NSE), a marker of small cell lung cancer (SCLC), as a targeted analyte. With optimal requirements, the configuration can provide a detection range of 10 pg/mL to 50 ng/mL and a detection limit of 7.4 pg/mL, accompanied by sufficient practical analytical performance. Collectively, this paradigm provides a deeper understanding of the ECL characteristics of coordination-driven J-aggregation and provides more possibilities for the development of ECL patterns based on luminescent metal–organic frameworks.

中文翻译:

配位驱动的 J-聚集体 K-PTC MOF 的高性能电化学发光,用于生物标记物分析

通过金属-配体配位消除聚集引起的多环芳烃猝灭在固态电化学发光 (ECL) 传感中具有巨大的科学意义。这里的钾离子(K +) 介导的 J-聚集体 K-PTC MOF(PTCA,perylene-3,4,9,10-tetracarboxylic)被合成并用于配制用于生物标志物检测的 ECL 免疫传感器。协调驱动的聚集体以端到端的侧模式排列,克服了与 PTCA 浓度相关的聚集引起的猝灭。与 PTCA 相比,K-PTC MOF 显示出更稳定的 ECL 发射,具有前所未有的红移至 718 nm,并且在 -1.1 V 的电压下具有用于分析应用的 ECL 活性。考虑到精确检测的要求,金属酚生物活性合成纳米粒子 (MPNPs) 用于构建夹层传感平台,以实现 ECL 的稳态调节。作为适用性的证明,使用神经元特异性烯醇化酶 (NSE) 进行了建设性实验,作为靶向分析物,小细胞肺癌 (SCLC) 的标志物。在最佳要求下,该配置可提供10 pg/mL至50 ng/mL的检测范围和7.4 pg/mL的检测限,并具有足够的实用分析性能。总的来说,这种范式提供了对协调驱动的 J 聚集的 ECL 特征的更深入理解,并为基于发光金属-有机框架的 ECL 模式的开发提供了更多可能性。
更新日期:2022-12-19
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