Abstract. Amino sugars, as the biomarkers of microbial residues, help explore soil microorganisms’ response to global climate change. However, research on how microorganisms in the alpine meadows regulate the soil carbon cycle under the influence of climate change is limited. We hypothesized that climate change might cause different effects on soil microbial residues due to the impact on soil physical properties, chemical properties, and enzyme activities. Therefore, we conducted four-year continuous warming and increased precipitation experiments in the semi-arid grasslands to examine the differences in soil microbial residues at different depths under simulated changes. The results showed warming stimulated the accumulation of microbial residues, while increased precipitation led to their decline. The Glucosamine/Muramic Acid ratio trend indicates that the contribution of fungal residues was more significant than that of bacteria with increased precipitation, whereas that of bacterial residues exceeded that of fungi with warming. The increased precipitation had no significant effect on soil extracellular enzyme activities and amino sugar concentrations. In addition, changes in the different enzyme activities led to soil carbon loss and different amino sugar accumulation patterns under the warming treatment. These results may aid in assessing the responses of soil microorganisms to future climate change scenarios.

中文翻译：

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变暖和降水增加对青藏高寒草甸土壤微生物残留的影响

摘要。氨基糖作为微生物残留物的生物标志物，有助于探索土壤微生物对全球气候变化的响应。然而，关于气候变化影响下高寒草甸微生物如何调控土壤碳循环的研究还很有限。由于气候变化对土壤物理性质、化学性质和酶活性的影响，我们假设气候变化可能对土壤微生物残留物产生不同的影响。为此，我们在半干旱草地进行了为期四年的连续增温增降水实验，考察模拟变化下不同深度土壤微生物残留的差异。结果表明，变暖刺激了微生物残留物的积累，而降水增加导致它们减少。氨基葡萄糖/胞壁酸比值趋势表明，随着降水增加，真菌残留的贡献比细菌的贡献更显着，而随着变暖，细菌残留的贡献超过真菌。降水增加对土壤胞外酶活性和氨基糖浓度没有显着影响。此外，不同酶活性的变化导致土壤碳损失和增温处理下不同的氨基糖积累模式。这些结果可能有助于评估土壤微生物对未来气候变化情景的反应。降水增加对土壤胞外酶活性和氨基糖浓度没有显着影响。此外，不同酶活性的变化导致土壤碳损失和增温处理下不同的氨基糖积累模式。这些结果可能有助于评估土壤微生物对未来气候变化情景的反应。降水增加对土壤胞外酶活性和氨基糖浓度没有显着影响。此外，不同酶活性的变化导致土壤碳损失和增温处理下不同的氨基糖积累模式。这些结果可能有助于评估土壤微生物对未来气候变化情景的反应。