Multifunctional wound dressings deliver numerous complementary therapeutics in a single, easy-to-use material; however, incompatibilities between additives and the inability to control spatial loading (i.e., surface vs. bulk) sometimes complicate material design and inhibit the use of all available beneficial additives. 3D printing affords the capability to fabricate polymeric wound dressings in unique and individually specific morphologies, as well as the ability to control spatial composition of the multifunctional wound dressing to overcome some of the aforementioned shortfalls from traditional material fabrication techniques. We demonstrate 3D printing as a viable tool to place additives from novel class of antimicrobials, cyclic peptides, in a UV curable, and multifunctional (absorbent and hemostatic) microporous polymer. Microscopic and material characterization confirmed loading of cyclic peptide and microporous architecture was unaffected by peptide loading and the extrusion 3D printing process. Cytotoxic evaluations indicate toxicity of peptide additives toward prokaryotic and eukaryotic cells and highlight the importance of spatial control afforded by 3D printing. The robustness of high internal phase emulsion templating and 3D printing was confirmed with antimicrobial assays that showed antimicrobial efficacy of the cyclic peptides were maintained and unaffected by polymerization and processing.

中文翻译：

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用于抗菌伤口敷料材料的 3D 可打印环肽负载微孔聚合物

多功能伤口敷料在单一、易于使用的材料中提供多种辅助疗法；然而，添加剂之间的不相容性和无法控制空间载荷（即表面与体积）有时会使材料设计复杂化并抑制所有可用的有益添加剂的使用。3D 打印提供了制造具有独特和单独特定形态的聚合物伤口敷料的能力，以及控制多功能伤口敷料的空间组成的能力，以克服传统材料制造技术的上述一些不足。我们展示了 3D 打印作为一种可行的工具，可以将来自新型抗菌剂、环肽的添加剂置于紫外线固化和多功能（吸收和止血）微孔聚合物中。显微和材料表征证实环肽的负载和微孔结构不受肽负载和挤出 3D 打印过程的影响。细胞毒性评估表明肽添加剂对原核和真核细胞的毒性，并强调了 3D 打印提供的空间控制的重要性。高内相乳液模板和 3D 打印的稳健性通过抗菌测定得到证实，表明环肽的抗菌功效得到维持并且不受聚合和加工的影响。细胞毒性评估表明肽添加剂对原核和真核细胞的毒性，并强调了 3D 打印提供的空间控制的重要性。高内相乳液模板和 3D 打印的稳健性通过抗菌测定得到证实，表明环肽的抗菌功效得到维持并且不受聚合和加工的影响。细胞毒性评估表明肽添加剂对原核和真核细胞的毒性，并强调了 3D 打印提供的空间控制的重要性。高内相乳液模板和 3D 打印的稳健性通过抗菌测定得到证实，表明环肽的抗菌功效得到维持并且不受聚合和加工的影响。