The glutathione (GSH) functionalized Mn-doped ZnS quantum dots (GSH_Mn_ZnS QDs) was conjugated with pyridoxal 5’-phosphate (PLP). The –CHO group of vitamin B₆ cofactor PLP interacted with the -NH₂ group of GSH functionalized Mn_ZnS QDs. The conjugation of PLP quenched the fluorescence emission of GSH_Mn_ZnS QDs at 601 nm. Addition of alkaline phosphatase (ALP) catalytically dephosphorylated the PLP into pyridoxal that restored the fluorescence emission of GSH_Mn_ZnS QDs. With a sensitivity of 0.035 U/L, the PLP conjugated GSH_Mn_ZnS QDs was applied to quantify ALP activity in human serum and plasma. Further, the developed nanoprobe PLP conjugated GSH_Mn_ZnS QDs was also applied to detect Al³⁺. The complexation-induced fluorescence enhancement was observed at 492 nm upon the interaction of Al³⁺ with the PLP conjugated GSH_Mn_ZnS QDs. Without any interference from other tested metal ions, this nanoprobe can be employed to detect Al³⁺ down to 2.30 µM.

谷胱甘肽 (GSH) 功能化的 Mn 掺杂 ZnS 量子点 (GSH_Mn_ZnS QDs) 与吡哆醛 5'-磷酸 (PLP) 结合。_{维生素 B 6}辅助因子 PLP的 –CHO 组与GSH 功能化 Mn_ZnS QD 的-NH _{2组相互作用。}PLP 的共轭淬灭了 GSH_Mn_ZnS QD 在 601 nm 的荧光发射。添加碱性磷酸酶 (ALP) 可将 PLP 催化去磷酸化为吡哆醛，从而恢复 GSH_Mn_ZnS QD 的荧光发射。PLP 共轭 GSH_Mn_ZnS QD 的灵敏度为 0.035 U/L，用于量化人血清和血浆中的 ALP 活性。此外，开发的纳米探针PLP共轭GSH_Mn_ZnS QDs也用于检测Al ³⁺. ^{在 Al 3+}与 PLP 共轭 GSH_Mn_ZnS QD相互作用时，在 492 nm 观察到络合诱导的荧光增强。在不受其他测试金属离子干扰的情况下，该纳米探针可用于检测低至 2.30 µM 的 Al ^{3+ 。}