The kinetics of regulation of vesicle-inducing protein in plastids (Vipp1), an essential protein in all oxygenic phototrophs, are not completely understood. Vipp1 is responsible for both membrane maintenance and damage repair processes, aggregating at sites of damage (proton leakage) or physical strain (responding to presence of anionic lipids). Herein we spatiotemporally resolve exchange between inactive cytoplasmic Vipp1 and these two functionalities. We demonstrate that Vipp1 can be aggregated to a specific site in the thylakoid membrane by laser damage, but this behavior appears to be mediated by a rapidly resolved secondary process, as cytoplasmic Vipp1 is rendered unresponsive to nearby damage on a time scale three orders of magnitude faster than the aggregation process itself.

中文翻译：

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蓝藻中 Vipp1 聚集的动力学和靶向

质体中囊泡诱导蛋白 (Vipp1) 的调节动力学尚不完全清楚，Vipp1 是所有含氧光养生物中的一种必需蛋白。Vipp1 负责膜维护和损伤修复过程，聚集在损伤部位（质子泄漏）或物理应变（响应阴离子脂质的存在）。在这里，我们在时空上解决了非活性细胞质 Vipp1 与这两种功能之间的交换。我们证明 Vipp1 可以通过激光损伤聚集到类囊体膜中的特定位点，但这种行为似乎是由快速解决的二级过程介导的，因为细胞质 Vipp1 在三个数量级的时间尺度上对附近的损伤没有反应比聚合过程本身更快。