In this work, an enzymatic method for the de novo construction of diverse substituted pyrazoles from readily available diazo compounds and alkynoates/allenoates in ionic liquids was established. Under the optimum reaction condition (3-alkynoates or allenoates (0.5 mmol), diazo compounds (0.6 mmol), [BMIM][PF₆] (2 mL), C. rugosa lipase (15 mg), 45 °C, 8 h.), pyrazoles bearing different groups were obtained in satisfactory yields (61 %−93 %). The method features a diversity of substituents of the pyrazole products and remarkably simple work-up.

中文翻译：

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离子液体中脂肪酶催化[3+2]环加成高效合成取代吡唑

在这项工作中，建立了一种酶促方法，用于从离子液体中容易获得的重氮化合物和炔酸/联烯酸从头构建各种取代的吡唑。在最佳反应条件下（3-alkynoates or allenoates (0.5 mmol), diazo compounds (0.6 mmol), [BMIM][PF ₆ ] (2 mL), C. rugosa lipase (15 mg), 45 °C, 8 h .), 以令人满意的收率 (61 %−93 %) 获得了带有不同基团的吡唑。该方法的特点是吡唑产品的取代基多种多样，而且后处理非常简单。