NIR-II Dyad-Doped Ratiometric Nanosensor with Enhanced Spectral Fidelity in Biological Media for In Vivo Biosensing,Nano Letters

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NIR-II Dyad-Doped Ratiometric Nanosensor with Enhanced Spectral Fidelity in Biological Media for In Vivo Biosensing
Nano Letters ( IF 9.6 ) Pub Date : 2022-12-01 , DOI: 10.1021/acs.nanolett.2c04084
Peng Yu ₁ , Kui Yan ₁ , Shangfeng Wang ₁ , Chenzhi Yao ₁ , Zuhai Lei ₂ , Yaohui Tang ₃ , Fan Zhang ₁

Affiliation

Ratiometric fluorescence nanosensors provide quantitative biological information. However, spectral shift and distortion of ratiometric nanosensors in biological media often compromise sensing accuracy, limiting in vivo applications. Here, we develop a fluorescent dyad (aBOP-IR1110) in the second near-infrared (NIR-II) window by covalently linking an asymmetric aza-BODIPY with a ONOO^–-responsive meso-thiocyanine. The dyad encapsulated in the PEGylated nanomicelle largely improves spectral fidelity in serum culture by >9.4 times compared to that of its noncovalent counterpart. The increased molecular weights (>1480 Da) and hydrophobicity (LogP of 7.87–12.36) lock dyads inside the micelles, which act as the shield against the external environment. ONOO^–-altered intramolecular Förster resonance energy transfer (FRET) generates linear ratiometric response with better serum tolerance, enabling us to monitor the dynamics of oxidative stress in traumatic brain injury and evaluate therapeutic efficiency. The results show high correlation with in vitro triphenyltetrazolium chloride staining, suggesting the potential of NIR-II dyad-doped nanosensor for in vivo high-fidelity sensing applications.

中文翻译：

NIR-II Dyad-Doped 比例纳米传感器，在生物介质中具有增强的光谱保真度，用于体内生物传感

比例荧光纳米传感器提供定量的生物信息。然而，生物介质中比例纳米传感器的光谱偏移和失真通常会影响传感精度，从而限制体内应用。在这里，我们在第二个近红外 (NIR-II) 窗口中通过共价连接不对称的氮杂 BODIPY 与 ONOO ^-响应性内消旋硫氰酸开发了荧光二元组 ( aBOP-IR1110 ) 。与非共价对应物相比，封装在聚乙二醇化纳米胶束中的二元体大大提高了血清培养物中的光谱保真度 > 9.4 倍。增加的分子量 (>1480 Da) 和疏水性 (Log P7.87–12.36) 将二元组锁定在胶束内，充当抵御外部环境的屏障。ONOO——改变^的分子内 Förster 共振能量转移 (FRET) 产生具有更好血清耐受性的线性比例反应，使我们能够监测创伤性脑损伤中氧化应激的动态并评估治疗效果。结果显示与体外三苯基氯化四唑染色高度相关，表明 NIR-II 二元掺杂纳米传感器在体内高保真传感应用中的潜力。

更新日期：2022-12-01

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