Maternal Zearalenone Exposure Affects Gut Microbiota and Follicular Development in Suckled Offspring,Journal of Agricultural and Food Chemistry

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Maternal Zearalenone Exposure Affects Gut Microbiota and Follicular Development in Suckled Offspring
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-12-01 , DOI: 10.1021/acs.jafc.2c06457
Jiamao Yan ₁ , Li Kong _{1,

2} , Xiaoyuan Zhang ₁ , Mubin Yu ₁ , Kexin Zhu ₁ , Aihong Zhao ₃ , Dachuan Shi ₃ , Yonghong Sun ₃ , Junjie Wang ₁ , Wei Shen ₁ , Lan Li ₁

Affiliation

Zearalenone (ZEN) is a mycotoxin that is widely present in feed and agricultural products. Studies have demonstrated that ZEN, as a type of estrogen analogue, can significantly affect the female reproductive system. Breast milk is the best nutrient for infant growth and development, but it is still unknown whether ZEN influences the fertility of offspring through suckling. In this study, we collected fecal and ovarian tissue from neonatal female offspring, whose mothers were exposed to ZEN for 21 days, and explored the effects of maternal ZEN exposure on intestinal microecology and follicular development in the mouse using 16S rRNA amplicon sequencing technology. Our findings suggested that maternal ZEN exposure significantly diminished ovarian reserve, increased apoptosis of ovarian granulosa cell (GC), and impacted the developmental competence of oocytes in lactating offspring. In addition, the results of 16S rRNA sequencing showed that the abundance of gut microbiota in offspring was significantly changed, including Bacteroidetes, Proteobacteria, and Firmicutes. This leads to alterations of glutathione metabolism and the expression of antioxidant enzymes in ovaries. In summary, our findings supported a potential relationship between gut microbiota and abnormal ovarian development caused by ZEN, which offers novel insights for therapeutic strategies for reproductive disorders induced by ZEN exposure.

中文翻译：

母体暴露于玉米赤霉烯酮会影响哺乳后代的肠道微生物群和卵泡发育

玉米赤霉烯酮 (ZEN) 是一种霉菌毒素，广泛存在于饲料和农产品中。研究表明，ZEN 作为一种雌激素类似物，可以显着影响女性生殖系统。母乳是婴儿生长发育最好的营养物质，但ZEN是否通过哺乳影响后代的生育能力尚不清楚。在这项研究中，我们收集了母体暴露于 ZEN 21 天的新生雌性后代的粪便和卵巢组织，并利用 16S rRNA 扩增子测序技术探讨了母体 ZEN 暴露对小鼠肠道微生态和卵泡发育的影响。我们的研究结果表明，母体 ZEN 暴露显着降低了卵巢储备，增加了卵巢颗粒细胞 (GC) 的凋亡，并影响卵母细胞在哺乳期后代中的发育能力。此外，16S rRNA测序结果显示，后代肠道菌群丰度发生显着变化，包括拟杆菌门、变形菌门和厚壁菌门。这导致谷胱甘肽代谢的改变和卵巢中抗氧化酶的表达。总之，我们的研究结果支持肠道微生物群与 ZEN 引起的卵巢异常发育之间的潜在关系，这为 ZEN 暴露引起的生殖障碍的治疗策略提供了新的见解。

更新日期：2022-12-01

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