The Heilongjiang brown frog (Rana amurensis) is widely used in traditional Chinese medicine. In particular, the oviduct and skin have been developed into various health products. However, limited numbers of complete genomes of amphibian species have been reported, excluding the Heilongjiang brown frog. Here, the transcriptomes of 45 samples from the liver, spleen, heart, ovaries, thigh muscles, skin, oviduct, stomach and intestine of five Heilongjiang brown frog were reassembled and analyzed. A total of 1,085,532 unigenes with an average length of 676.6 bp and N50 of 722 bp were obtained. Comparative transcriptomics of different tissues detected tissue-specific expression. There were 3248 differentially expressed genes (DEGs) in the ovary, and the number of unique DEGs between the ovary and spleen was the largest. The results of DEGs enrichment showed there were many pathways and items related to protein synthesis and metabolism in the oviduct. The DEGs of the skin were enriched with many bacterial defense items, indicating that there were a large number of antimicrobial peptides in the skin. Thus, these were suitable as biological sources for the development and extraction of antimicrobial peptides. Through the assembly of transcriptome sequencing data and functional annotation of the Heilongjiang brown frog genome, this study provides reference materials for further exploring and utilizing functional gene resources of frogs and lays a foundation for medical research and the development of new products.

黑龙江褐蛙（Rana amurensis)广泛用于中药。特别是输卵管和皮肤已被开发成各种保健品。然而，已报道的两栖动物物种的完整基因组数量有限，不包括黑龙江褐蛙。在这里，对来自五只黑龙江褐蛙的肝脏、脾脏、心脏、卵巢、大腿肌肉、皮肤、输卵管、胃和肠的 45 个样本的转录组进行了重组和分析。共获得1,085,532个unigenes，平均长度为676.6 bp，N50为722 bp。不同组织的比较转录组学检测到组织特异性表达。卵巢中有3248个差异表达基因（DEG），其中卵巢和脾脏之间的独特DEG数量最多。DEGs富集结果表明，输卵管中存在许多与蛋白质合成和代谢相关的通路和项目。皮肤的 DEGs 富含许多细菌防御项目，表明皮肤中存在大量的抗菌肽。因此，这些适合作为开发和提取抗菌肽的生物来源。本研究通过黑龙江褐蛙基因组转录组测序数据的组装和功能注释，为进一步挖掘和利用蛙类功能基因资源提供参考资料，为医学研究和新产品开发奠定基础。表明皮肤中存在大量的抗菌肽。因此，这些适合作为开发和提取抗菌肽的生物来源。本研究通过黑龙江褐蛙基因组转录组测序数据的组装和功能注释，为进一步挖掘和利用蛙类功能基因资源提供参考资料，为医学研究和新产品开发奠定基础。表明皮肤中存在大量的抗菌肽。因此，这些适合作为开发和提取抗菌肽的生物来源。本研究通过黑龙江褐蛙基因组转录组测序数据的组装和功能注释，为进一步挖掘和利用蛙类功能基因资源提供参考资料，为医学研究和新产品开发奠定基础。