Transfusion of healthy red blood cells (RBCs) is a lifesaving process. However, upon storing RBCs, a wide range of damage-associate molecular patterns (DAMPs), such as cell-free DNA, nucleosomes, free-hemoglobin, and poly-unsaturated-fatty-acids are generated. DAMPs can further damage RBCs; thus, the quality of stored RBCs declines during the storage and limits their shelf-life. Since these DAMPs consist of either positive or negative charged species, we developed taurine and acridine containing electrospun-nanofibrous-sheets (Tau-AcrNFS), featuring anionic, cationic charges and an DNA intercalating group on their surfaces. We show that Tau-AcrNFS are efficient in scavenging DAMPs from stored human and mice RBCs ex vivo. We find that intermittent scavenging of DAMPs by Tau-AcrNFS during the storage reduces the loss of RBC membrane integrity and reduces discocytes-to-spheroechinocytes transformation in stored-old-RBCs. We perform RBC-transfusion studies in mice to reveal that intermittent removal of DAMPs enhances the quality of stored-old-RBCs equivalent to freshly collected RBCs, and increases their shelf-life by ~22%. Such prophylactic technology may lead to the development of novel blood bags or medical device, and may therefore impact healthcare by reducing transfusion-related adverse effects.

输入健康的红细胞 (RBC) 是一个挽救生命的过程。然而，在储存 RBC 时，会产生范围广泛的损伤相关分子模式 (DAMP)，例如无细胞 DNA、核小体、游离血红蛋白和多不饱和脂肪酸。DAMP 会进一步损害红细胞；因此，储存的红细胞质量在储存期间下降并限制了它们的保质期。由于这些 DAMP 由带正电或带负电的物种组成，我们开发了含有牛磺酸和吖啶的电纺纳米纤维片 ( Tau-Acr NFS)，其表面具有阴离子、阳离子电荷和 DNA 嵌入基团。我们表明，Tau-Acr NFS 可以有效地从体外储存的人和小鼠红细胞中清除 DAMP。我们发现间歇性清除 DAMPTau - Acr NFS 在储存期间减少了红细胞膜完整性的损失，并减少了储存的旧红细胞中盘细胞向球棘细胞的转化。我们对小鼠进行红细胞输注研究，揭示间歇性去除 DAMP 可提高储存的旧红细胞的质量，相当于新收集的红细胞，并将其保质期延长约 22%。这种预防技术可能会导致新型血袋或医疗设备的开发，因此可能会通过减少与输血相关的不良反应来影响医疗保健。