Persistent usage of pesticides in agriculture has posed serious damage to overall ecosystem and human health, and thereby it is imperative to develop sensitive and efficient tools to evaluate residual pesticides in food and environmental setting. Herein, we reported a switchable colorimetric probe toward fipronil residue sensitized with aptamer-fueled catalytic activity of affiliative ZIF-8. Innovatively, it was found that the attached aptamer preferred to adsorb 3,3′,5,5′-tetramethylbenzidine (TMB) rather than 2,2-azinobis (3-ethylbenzothiazo-line-6-sulfonic acid) (ABTS), greatly promoting catalytic oxidation of ZIF-8 toward TMB for further improving sensitivity. Aiding with smartphone-based image acquisition, fipronil-responsive discoloration degree was converted into the ratio of green and blue (G/B) with limit of detection as low as 0.036 μM (0.016 μg·g⁻¹). Moreover, it allowed for fipronil analysis in water, soil and vegetable samples with good recovery between 87 % and 110 %, verifying extension application prospect of the aptamer-fueled colorimetry for on-field pesticide evaluation in food safety supervision.

农业中持续使用农药对整体生态系统和人类健康造成严重损害，因此必须开发灵敏有效的工具来评估食品和环境环境中的残留农药。在此，我们报道了一种可切换的比色探针，用于对氟虫腈残留物敏感，该残留物对附属 ZIF-8 的适体催化活性敏感。创新性地发现，附着的适配体更喜欢吸附 3,3',5,5'-四甲基联苯胺 (TMB) 而不是 2,2-偶氮基双（3-乙基苯并噻唑啉-6-磺酸）(ABTS)，大大促进 ZIF-8 对 TMB 的催化氧化，进一步提高灵敏度。借助基于智能手机的图像采集，氟虫腈响应变色度被转换为绿色和蓝色比率 (G/B)，检测限低至 0。^-1）。此外，它还可以对水、土壤和蔬菜样品进行氟虫腈分析，回收率在 87% 至 110% 之间，验证了适配体燃料比色法在食品安全监管中农药现场评价的扩展应用前景。