Herein, a novel immunochromatographic assay (ICA) based on metal–organic framework-decorated polydopamine (MOF@PDA) was firstly developed for the determination of hydrochlorothiazide (HCTZ) adulteration in functional foods. The coupling rate of MOF@PDA carrier to HCTZ antibody was as high as 91.7 %. The detection limits of the developed MOF@PDA-ICA in functional tablets and capsules were 5.93 and 4.72 μg/kg, the linear ranges were 11.2–91.91 μg/kg and 9.11–86.78 μg/kg, respectively. The sensitivity was 27-fold higher than that of the reported ICA. The recovery was 82.5–116.6 %, and coefficient of variation was 6.9–14.2 %. The results can be achieved and analyzed in 8 min with the smartphone-based detection device. The parallel tests of 23 commercial functional tablets and capsules showed that the results of the MOF@PDA-ICA were consistent with that of the LC-MS/MS (R² > 0.99). Therefore, our method is facile, sensitive, portable, and can provide a reliable technical mean for the detection of HCTZ adulteration in functional foods.

在此，首次开发了一种基于金属有机骨架修饰的聚多巴胺（MOF@PDA）的新型免疫层析法（ICA），用于测定功能性食品中氢氯噻嗪（HCTZ）的掺假。MOF@PDA载体与HCTZ抗体的偶联率高达91.7%。开发的 MOF@PDA-ICA 在功能性片剂和胶囊中的检测限分别为 5.93 和 4.72 μg/kg，线性范围分别为 11.2–91.91 μg/kg 和 9.11–86.78 μg/kg。灵敏度比报道的 ICA 高 27 倍。回收率为 82.5–116.6%，变异系数为 6.9–14.2%。使用基于智能手机的检测设备可以在 8 分钟内获得结果并进行分析。²  > 0.99)。因此，该方法简便、灵敏、便携，可为检测功能性食品中HCTZ掺假提供可靠的技术手段。