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Facile immunochromatographic assay based on metal–organic framework-decorated polydopamine for the determination of hydrochlorothiazide adulteration in functional foods
Food Chemistry ( IF 8.5 ) Pub Date : 2022-11-29 , DOI: 10.1016/j.foodchem.2022.135100
Zhiwei Liu 1 , Zixian He 1 , Jinxiao Wu 2 , Houliang Lin 1 , Youwen Deng 1 , Xing Shen 1 , Hongtao Lei 1 , Xiangmei Li 1
Affiliation  

Herein, a novel immunochromatographic assay (ICA) based on metal–organic framework-decorated polydopamine (MOF@PDA) was firstly developed for the determination of hydrochlorothiazide (HCTZ) adulteration in functional foods. The coupling rate of MOF@PDA carrier to HCTZ antibody was as high as 91.7 %. The detection limits of the developed MOF@PDA-ICA in functional tablets and capsules were 5.93 and 4.72 μg/kg, the linear ranges were 11.2–91.91 μg/kg and 9.11–86.78 μg/kg, respectively. The sensitivity was 27-fold higher than that of the reported ICA. The recovery was 82.5–116.6 %, and coefficient of variation was 6.9–14.2 %. The results can be achieved and analyzed in 8 min with the smartphone-based detection device. The parallel tests of 23 commercial functional tablets and capsules showed that the results of the MOF@PDA-ICA were consistent with that of the LC-MS/MS (R2 > 0.99). Therefore, our method is facile, sensitive, portable, and can provide a reliable technical mean for the detection of HCTZ adulteration in functional foods.



中文翻译:

基于金属有机骨架修饰的聚多巴胺的简易免疫层析法测定功能性食品中氢氯噻嗪的掺假

在此,首次开发了一种基于金属有机骨架修饰的聚多巴胺(MOF@PDA)的新型免疫层析法(ICA),用于测定功能性食品中氢氯噻嗪(HCTZ)的掺假。MOF@PDA载体与HCTZ抗体的偶联率高达91.7%。开发的 MOF@PDA-ICA 在功能性片剂和胶囊中的检测限分别为 5.93 和 4.72 μg/kg,线性范围分别为 11.2–91.91 μg/kg 和 9.11–86.78 μg/kg。灵敏度比报道的 ICA 高 27 倍。回收率为 82.5–116.6%,变异系数为 6.9–14.2%。使用基于智能手机的检测设备可以在 8 分钟内获得结果并进行分析。2  > 0.99)。因此,该方法简便、灵敏、便携,可为检测功能性食品中HCTZ掺假提供可靠的技术手段。

更新日期:2022-12-02
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