The aim of current study was to optimize the extraction process and purification of main components (MC) to obtain high antioxidant, anti-inflammatory effective fractions from crabapple (Malus prunifolia (Willd.) Borkh.). The effects of three variables including ethanol concentration A₁, solid-liquid ratio A₂, extraction temperature A₃ were investigated and optimized by response surface methodology (RSM) coupled with Box-Behnken design (BBD). The adsorption/desorption characteristics of MC on the five types of macroporous resins were investigated. According to batch adsorption test, HPD-300 resins were selected for kinetics. The adsorption mechanism showed that the process was appropriate by pseudo-second-order kinetics model, and purification parameters of MC were optimized through adsorption/desorption experiments with the column packed by HPD-300 resin. The effective fractions were obviously superior to other fractions according to DPPH, ABTS, COX-2 and 15-LOX radical scavenging. This work implies that the purified active fraction with high contents of antioxidants and anti-inflammatory compounds from crabapple might be potential source for natural products and food industries.

本研究的目的是优化主要成分 (MC) 的提取工艺和纯化，从海棠 ( Malus prunifolia (Willd.) Borkh.)中获得高抗氧化、抗炎有效部位。乙醇浓度A ₁、料液比A ₂、提取温度A _{3三个变量的影响}通过响应面法 (RSM) 结合 Box-Behnken 设计 (BBD) 进行调查和优化。研究了 MC 在五种大孔树脂上的吸附/解吸特性。根据批量吸附试验，选择HPD-300树脂进行动力学分析。吸附机理表明该过程符合准二级动力学模型，并通过HPD-300树脂填充柱的吸附/解吸实验优化了MC的纯化参数。根据DPPH、ABTS、COX-2和15-LOX自由基清除，有效馏分明显优于其他馏分。