A Conformational Restriction Strategy for the Control of CRISPR/Cas Gene Editing with Photoactivatable Guide RNAs**,Angewandte Chemie International Edition

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A Conformational Restriction Strategy for the Control of CRISPR/Cas Gene Editing with Photoactivatable Guide RNAs**
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2022-12-01 , DOI: 10.1002/anie.202212413
Ying-Jie Sun ₁ , Wen-Da Chen _{1,

2} , Ji Liu ₃ , Jun-Jin Li ₄ , Yu Zhang ₅ , Wei-Qi Cai _{2,

3} , Li Liu _{1,

2} , Xin-Jing Tang ₅ , Jian Hou ₄ , Ming Wang _{2,

3} , Liang Cheng _{1,

2,

6}

Affiliation

We report on the discovery and development of conformationally restricted guide RNA, a head-to-tail cyclized molecule that can be easily prepared with only two or three modification sites. This is a superior approach for the spatiotemporal control of CRISPR/Cas editing, which has enabled the precise cleavage of exogenous genes at a predefined cutting region without notable editing leakages in live cells and in embryos.

中文翻译：

利用光激活向导 RNA 控制 CRISPR/Cas 基因编辑的构象限制策略**

我们报告了构象限制向导RNA的发现和开发，这是一种头尾相连的环化分子，只需两个或三个修饰位点即可轻松制备。这是一种用于 CRISPR/Cas 编辑时空控制的优越方法，它能够在预定义的切割区域精确切割外源基因，而不会在活细胞和胚胎中出现明显的编辑泄漏。

更新日期：2022-12-01

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