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A Conformational Restriction Strategy for the Control of CRISPR/Cas Gene Editing with Photoactivatable Guide RNAs**
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2022-12-01 , DOI: 10.1002/anie.202212413
Ying-Jie Sun 1 , Wen-Da Chen 1, 2 , Ji Liu 3 , Jun-Jin Li 4 , Yu Zhang 5 , Wei-Qi Cai 2, 3 , Li Liu 1, 2 , Xin-Jing Tang 5 , Jian Hou 4 , Ming Wang 2, 3 , Liang Cheng 1, 2, 6
Affiliation  

We report on the discovery and development of conformationally restricted guide RNA, a head-to-tail cyclized molecule that can be easily prepared with only two or three modification sites. This is a superior approach for the spatiotemporal control of CRISPR/Cas editing, which has enabled the precise cleavage of exogenous genes at a predefined cutting region without notable editing leakages in live cells and in embryos.

中文翻译:

利用光激活向导 RNA 控制 CRISPR/Cas 基因编辑的构象限制策略**

我们报告了构象限制向导RNA的发现和开发,这是一种头尾相连的环化分子,只需两个或三个修饰位点即可轻松制备。这是一种用于 CRISPR/Cas 编辑时空控制的优越方法,它能够在预定义的切割区域精确切割外源基因,而不会在活细胞和胚胎中出现明显的编辑泄漏。
更新日期:2022-12-01
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