Deciphering the Myrosinase-like Activity of Lactiplantibacillus plantarum WCFS1 among GH1 Family Glycoside Hydrolases,Journal of Agricultural and Food Chemistry

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Deciphering the Myrosinase-like Activity of Lactiplantibacillus plantarum WCFS1 among GH1 Family Glycoside Hydrolases
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-12-01 , DOI: 10.1021/acs.jafc.2c06240
Laura Plaza-Vinuesa ₁ , Oswaldo Hernandez-Hernandez ₂ , Ana Sánchez-Arroyo ₁ , José M Cumella ₃ , Nieves Corzo ₂ , Ana M Muñoz-Labrador ₂ , F Javier Moreno ₂ , Blanca de Las Rivas ₁ , Rosario Muñoz ₁

Affiliation

The hydrolysis of plant glucosinolates by myrosinases (thioglucosidases) originates metabolites with chemopreventive properties. In this study, the ability to hydrolyze the glucosinolate sinigrin by cultures or protein extracts of Lactiplantibacillus plantarum WCFS1 was assayed. This strain possesses myrosinase-like activity as sinigrin was partly hydrolyzed by induced cultures but not by protein extracts. The 11 glycoside hydrolase GH1 family proteins, annotated as 6-phospho-β-glucosidases, were the proteins most similar to plant myrosinases. The activity of these proteins was assayed against sinigrin and synthetic glucosides. As expected, none of the proteins assayed possessed myrosinase activity against sinigrin or the synthetic β-thio-glucoside derivative or against the β-glucoside. However, all 11 proteins were active on the phosphorylated-β-glucoside derivative. Moreover, only eight of these proteins were active on phospho-β-thioglucose. These results supported that, in L. plantarum WCFS1, glucosinolates may undergo previous phosphorylation, and GH1 proteins are the glycosidases involved in the hydrolysis of phosphorylated glucosinolates.

中文翻译：

破译植物乳杆菌 WCFS1 在 GH1 家族糖苷水解酶中的黑芥子酶样活性

黑芥子酶（硫葡糖苷酶）对植物芥子油苷的水解产生具有化学预防特性的代谢物。在这项研究中，通过植物乳杆菌的培养物或蛋白质提取物水解硫代葡萄糖苷的能力检测了 WCFS1。该菌株具有黑芥子酶样活性，因为黑芥子苷被诱导培养物部分水解，但不被蛋白质提取物水解。11 种糖苷水解酶 GH1 家族蛋白，注释为 6-磷酸-β-葡萄糖苷酶，是与植物黑芥子酶最相似的蛋白。这些蛋白质的活性是针对黑豆苷和合成糖苷进行测定的。正如预期的那样，所检测的蛋白质均不具有黑芥子酶活性以对抗黑芥子苷或合成的 β-硫代-葡糖苷衍生物或抗 β-葡糖苷。然而，所有 11 种蛋白质都对磷酸化-β-葡萄糖苷衍生物具有活性。此外，这些蛋白质中只有八种对磷酸-β-硫葡萄糖有活性。这些结果支持，在L. plantarumWCFS1，芥子油苷可能经历先前的磷酸化，GH1蛋白是参与磷酸化芥子油苷水解的糖苷酶。

更新日期：2022-12-01

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