Lipase-Entrapped Colloidosomes with Tunable Positioning at the Oil–Water Interface for Pickering Emulsion-Enhanced Biocatalysis,ACS Applied Materials & Interfaces

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Lipase-Entrapped Colloidosomes with Tunable Positioning at the Oil–Water Interface for Pickering Emulsion-Enhanced Biocatalysis
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2022-12-01 , DOI: 10.1021/acsami.2c17451
Qi Zeng ₁ , Mengmeng Sun ₁ , Xin Xie ₁ , Yuli Zhang ₁ , Haoyue Hou ₁ , Xingyuan Fang ₁ , Ting Guo ₁ , Hao Yuan ₁ , Tao Meng ₁

Affiliation

Pickering interfacial biocatalysis (PIB) paves the way for efficient enzymatic catalysis in the biphasic system. However, the Pickering interfacial biocatalysts located on the oil–water interface still face the inevitable deactivation when one of the phases contains the reactant that inactivates the enzyme. Herein, the positioning of lipase-entrapped colloidosomes (LECs) at the emulsion interface is rationally designed by physically tuning the wettability, which allows LECs to protrude into the selected phase to protect the lipase away from the damage of the reactant. As a proof of concept, LECs with different positioning at the interface are used as Pickering interfacial biocatalysts to produce biodiesel by esterification of lauric acid and methanol. Impressively, the LECs that protrude into the oil phase possess an optimal catalytic performance to protect more lipases away from the damage of the reactant of short-chain alcohol, which shows an 8.18-fold enhancement in specific activity relative to the free lipase, reach a biodiesel yield of 80.37% after 8 h, and retain the 96.44% of relative activity after 10 cycles. This study provides a novel and robust platform for Pickering emulsion-enhanced biocatalysis.

中文翻译：

脂肪酶包裹的胶体体在油水界面的可调定位用于皮克林乳液增强的生物催化

皮克林界面生物催化 (PIB) 为双相系统中的高效酶催化铺平了道路。然而，当其中一相含有使酶失活的反应物时，位于油水界面的皮克林界面生物催化剂仍然面临不可避免的失活。在此，通过物理调节润湿性合理设计脂肪酶包裹的胶体体（LECs）在乳液界面的定位，使 LECs 突出到选定的相中，以保护脂肪酶免受反应物的损害。作为概念验证，在界面处具有不同定位的 LEC 被用作皮克林界面生物催化剂，通过月桂酸和甲醇的酯化反应生产生物柴油。令人印象深刻的是，突入油相的LECs具有最佳的催化性能，保护更多的脂肪酶免受反应物短链醇的破坏，比活性比游离脂肪酶提高8.18倍，达到生物柴油收率8 h 后活性为 80.37%，10 个循环后仍保持 96.44% 的相对活性。本研究为皮克林乳液增强生物催化提供了一个新颖而稳健的平台。

更新日期：2022-12-01

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