Steric Hindrance On–Off Mass-Tagged Probe Set Enables Detection of Protein Homodimer in Living Cells,ACS Applied Materials & Interfaces

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Steric Hindrance On–Off Mass-Tagged Probe Set Enables Detection of Protein Homodimer in Living Cells
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2022-11-30 , DOI: 10.1021/acsami.2c15010
Xiaoxu Li ₁ , Jianhua Zhu ₁ , Xiaoyu Shi ₁ , Zhongcheng Wang ₁ , Xi Chen ₁ , Xian Zhang ₁ , Yun Chen _{1,

2,

3}

Affiliation

The major challenge in the detection of protein homodimers is that the identical monomers in a homodimer are indistinguishable using most conventional methods and cannot be sequentially recognized. In this study, a steric hindrance on–off mass-tagged probe set strategy was developed for the quantification of HER2 homodimer in living cells. The probe set contained a hindrance probe and a detection probe. The hindrance probe had a DNA dendrimer as a hindrance group to achieve the steric hindrance on–off function and thus the assignment of monomer identity. The detection probe contained a mass tag released for mass spectrometric quantification. Using the steric hindrance on–off mass-tagged probe set, the level of HER2 homodimer in various breast cancer cell lines was quantified. This is the first report to determine the quantity of protein homodimers, and the steric hindrance on–off probe set developed herein can facilitate the illustration of protein function in cancer.

中文翻译：

空间位阻开关质量标记探针组能够检测活细胞中的蛋白质同源二聚体

检测蛋白质同源二聚体的主要挑战是使用大多数常规方法无法区分同源二聚体中的相同单体，并且无法按顺序识别。在这项研究中，开发了空间位阻开关质量标记探针组策略，用于定量活细胞中的 HER2 同源二聚体。探针组包含一个阻碍探针和一个检测探针。位阻探针有一个 DNA 树枝状聚合物作为位阻基团，以实现空间位阻开关功能，从而分配单体身份。检测探针包含释放用于质谱定量的质量标签。使用空间位阻开关质量标记探针组，量化了各种乳腺癌细胞系中 HER2 同型二聚体的水平。这是第一份确定蛋白质同源二聚体数量的报告，

更新日期：2022-11-30

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