Qualitative and quantitative analysis of mercury at concentration levels as low as parts per billion (ppb) is a basic and practical concern. The vast majority of research in this field has centered on the development of potent chemosensor to monitor mercuric (Hg²⁺) ions. Mercury exists in three oxidation states, + 2, + 1 and 0, all of which are highly poisonous. In this study, (N¹E,N²E)-N¹,N²-bis(pyrene-1-ylmethylene)benzene-1,2-diamine (PAPM), a novel photoluminescent sensor based on pyrene platform was synthesized. Over the tested metal ions (Cd²⁺, Co²⁺, Cu²⁺, Mg²⁺, Mn²⁺, Ni²⁺, K⁺, Na⁺, Zn²⁺, Sr²⁺, Pb²⁺, Al³⁺, Cr³⁺ and Fe³⁺) the sensor responds only to Hg²⁺ by showing high selectivity and sensitivity. After treatment with mercuric ions at room temperature, the luminescence intensity of probe was quenched at 456 nm. The quenching of fluorescence intensity of probe upon addition of mercury is due to the effect of “turn-off” chelation enhanced quenching (CHEQ) by the formation of 1:1 complex. The ESI–MS spectrum and the Job’s experimental results confirm the formation of 1:1 complex between PAPM and Hg²⁺. The detection limit and association constant of sensor for mercury is computed using fluorescence titration data and were found to be 9.0 × 10^–8 M and 1.29 × 10⁵ M⁻¹ respectively. The practical application of sensor towards recognition of mercury(II) ions was explored through economically viable test strips and also using cell imaging studies.

在低至十亿分之一 (ppb) 的浓度水平下对汞进行定性和定量分析是一个基本且实际的问题。该领域的绝大多数研究都集中在开发有效的化学传感器以监测汞 (Hg ²⁺ ) 离子。汞以三种氧化态存在，+ 2、+ 1 和 0，它们都是剧毒的。本研究合成了( N ¹ E,N ² E )- N ¹ ,N ² -双(芘-1-基亚甲基)苯-1,2-二胺(PAPM)，一种基于芘平台的新型光致发光传感器。在被测金属离子（Cd ²⁺、Co ²⁺、Cu ²⁺、Mg ²⁺, Mn ²⁺ , Ni ²⁺ , K ⁺ , Na ⁺ , Zn ²⁺ , Sr ²⁺ , Pb ²⁺ , Al ³⁺ , Cr ³⁺和 Fe ³⁺ ) 传感器仅响应 Hg ²⁺显示高选择性和灵敏度。在室温下用汞离子处理后，探针的发光强度在 456 nm 处被淬灭。加入汞后探针荧光强度的猝灭是由于1:1络合物形成的“关闭”螯合增强猝灭（CHEQ）效应。ESI-MS 谱图和 Job 的实验结果证实了 PAPM 和 Hg ^{2+之间形成了 1:1 的复合物}. 利用荧光滴定数据计算出传感器对汞的检测限和缔合常数分别为9.0 × 10 –8 ^M 和1.29 × 10 ⁵  M ⁻¹。通过经济可行的试纸和细胞成像研究，探索了传感器在识别汞 (II) 离子方面的实际应用。