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Development of an Endoplasmic Reticulum-targeting Fluorescent Probe for the Imaging of Superoxide Anion in Living Cells
Journal of Fluorescence ( IF 2.6 ) Pub Date : 2022-11-30 , DOI: 10.1007/s10895-022-03079-x
Hua Wei 1 , Yan Wang 2 , Qingxian Chen 1 , Yaru Sun 1 , Tao Yue 2 , Baoli Dong 1
Affiliation  

Superoxide anion (O2•−) is an important reactive oxygen species (ROS), and plays critical roles in biological systems. ER stress has close relation with many metabolic diseases, and could lead to the abnormal production of ROS including O2•−. Herein, we present an ER-targeting probe (ER-Tf) for the detection of O2•− in living cells. The probe ER-Tf used triflate as the response site for O2•−, and employed p-methylbenzenesulfonamide as ER-targeting moiety. In response to O2•−, the triflate of the probe ER-Tf converted to hydroxyl group, providing strong blue emission under the excitation of ultraviolet light. The probe ER-Tf exhibited high sensitivity and selectivity to O2•−. Bioimaging experiments showed that the probe ER-Tf can be applied to detect O2•− at ER, and also demonstrated that rotenone could increase the generation of O2•− in living cells, while the O2•− level at ER showed no remarkable change during ferroptosis.



中文翻译:

用于活细胞超氧阴离子成像的内质网靶向荧光探针的开发

超氧阴离子(O 2 •− )是一种重要的活性氧(ROS),在生物系统中起着至关重要的作用。内质网应激与多种代谢性疾病密切相关,可导致包括O 2 •−在内的ROS异常产生。在此,我们提出了一种用于检测活细胞中O 2 •−的ER 靶向探针 ( ER-Tf )。探针ER-Tf使用三氟甲磺酸作为O 2 •−的响应位点,并使用对甲基苯磺酰胺作为ER靶向部分。响应 O 2 •−,探针ER-Tf的三氟甲磺酸转变为羟基,在紫外光的激发下发出强烈的蓝光。探针ER-Tf对O 2 •-表现出高灵敏度和选择性。生物成像实验表明, ER-Tf探针可用于检测ER处的O 2 •− ,同时也证明鱼藤酮可以增加活细胞中O 2 •−的生成,而ER处的O 2 •−水平显示没有ferroptosis 期间的显着变化。

更新日期:2022-12-01
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