Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy,Physical Review Letters

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Probing Cage Relaxation in Concentrated Protein Solutions by X-Ray Photon Correlation Spectroscopy
Physical Review Letters ( IF 8.1 ) Pub Date : 2022-11-29 , DOI: 10.1103/physrevlett.129.238001
Yuriy Chushkin ₁ , Alessandro Gulotta ₂ , Felix Roosen-Runge _{2,

3} , Antara Pal ₂ , Anna Stradner _{2,

4} , Peter Schurtenberger _{2,

4}

Affiliation

Diffusion of proteins on length scales of their size is crucial for understanding the machinery of living cells. X-ray photon correlation spectroscopy (XPCS) is currently the only way to access long-time collective diffusion on these length scales, but radiation damage so far limits the use in biological systems. We apply a new approach to use XPCS to measure cage relaxation in crowded

α

-crystallin solutions. This allows us to correct for radiation effects, obtain missing information on long time diffusion, and support the fundamental analogy between protein and colloid dynamical arrest.

中文翻译：

用 X 射线光子相关光谱法探测浓缩蛋白质溶液中的笼弛豫

蛋白质在其大小的长度尺度上的扩散对于理解活细胞的机制至关重要。X 射线光子相关光谱 (XPCS) 目前是在这些长度尺度上获取长期集体扩散的唯一方法，但辐射损伤迄今为止限制了在生物系统中的使用。我们应用了一种新方法，使用 XPCS 来测量拥挤环境中的笼子松弛度