Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis,Environmental Science & Technology

当前位置： X-MOL 学术 › Environ. Sci. Technol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Bisphenols Promote the Pheromone-Responsive Plasmid-Mediated Conjugative Transfer of Antibiotic Resistance Genes in Enterococcus faecalis
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2022-11-29 , DOI: 10.1021/acs.est.2c05349
Yutong Yang ₁ , Xiaobo Yang ₁ , Hongrui Zhou ₁ , Yuanyuan Niu _{1,

2} , Jing Li _{1,

3} , Xinyue Fu _{1,

2} , Shang Wang ₁ , Bin Xue ₁ , Chenyu Li ₁ , Chen Zhao ₁ , Xi Zhang ₁ , Zhiqiang Shen ₁ , Jingfeng Wang ₁ , Zhigang Qiu ₁

Affiliation

The enrichment and spread of antibiotic resistance genes (ARGs) induced by environmental chemical pollution further exacerbated the threat to human health and ecological safety. Several compounds are known to induce R plasmid-mediated conjugation through inducing reactive oxygen species (ROS), increasing cell membrane permeability, enhancing regulatory genes expression, and so forth. Up to now, there has been no substantial breakthrough in the studies of models and related mechanisms. Here, we established a new conjugation model using pheromone-responsive plasmid pCF10 and confirmed that five kinds of bisphenols (BPs) at environmentally relevant concentrations could significantly promote the conjugation of ARGs mediated by plasmid pCF10 in E. faecalis by up to 4.5-fold compared with untreated cells. Using qPCR, gene knockout and UHPLC, we explored the mechanisms behind this phenomenon using bisphenol A (BPA) as a model of BPs and demonstrated that BPA could upregulate the expression of pheromone, promote bacterial aggregation, and even directly activate conjugation as a pheromone instead of producing ROS and enhancing cell membrane permeability. Interestingly, the result of mathematical analysis showed that the pheromone effect of most BPs is more potent than that of synthetic pheromone cCF10. These findings provide new insight into the environmental behavior and biological effect of BPs and provided new method and theory to study on enrichment and spread of ARGs induced by environmental chemical pollution.

中文翻译：

双酚促进粪肠球菌中信息素响应质粒介导的抗生素抗性基因的结合转移

环境化学污染引起的抗生素抗性基因（antibiotic resistance genes, ARGs）的富集和扩散进一步加剧了对人类健康和生态安全的威胁。已知有几种化合物通过诱导活性氧 (ROS)、增加细胞膜通透性、增强调节基因表达等来诱导 R 质粒介导的结合。迄今为止，模型及相关机制的研究尚未取得实质性突破。在这里，我们使用信息素反应质粒 pCF10 建立了一个新的结合模型，并证实了环境相关浓度的五种双酚 (BPs) 可以显着促进质粒 pCF10 介导的 ARGs 在粪肠球菌中的结合与未处理的细胞相比高达 4.5 倍。使用 qPCR、基因敲除和 UHPLC，我们以双酚 A (BPA) 作为 BPs 的模型探索了这一现象背后的机制，并证明 BPA 可以上调信息素的表达，促进细菌聚集，甚至直接作为信息素激活结合产生 ROS 和增强细胞膜通透性。有趣的是，数学分析的结果表明，大多数 BPs 的信息素效应比合成信息素 cCF10 更有效。这些发现为BPs的环境行为和生物学效应提供了新的见解，为研究环境化学污染引起的ARGs的富集和扩散提供了新的方法和理论。

更新日期：2022-11-29

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11