Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat ( NB-LRR ) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height ( Rht ) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.

中文翻译：

---

自激活 NB-LRR 基因导致小麦 Rht13 侏儒症

半矮化基因在全球范围内大大提高了小麦产量，但广泛使用的赤霉素 (GA) 不敏感基因Rht-B1b和Rht-D1b对出苗不利。使用 GA 敏感的半矮化基因Rht13避免了这种多效性。在这里，我们表明Rht13编码一个核苷酸结合位点/富含亮氨酸重复序列(NB-LRR）基因。半矮人的点突变Rht-B13b等位基因自动激活NB-LRR基因并导致身高降低与Rht-B1b和Rht-D1b在不同的遗传背景下。自动激活Rht-B13b等位基因导致发病机制相关基因的转录上调，包括与细胞壁重塑相关的 III 类过氧化物酶。Rht13代表一类新的降低高度（右转) 基因，不同于其他右转基因，编码 GA 信号或代谢途径的成分。这一发现开辟了使用自动激活的途径NB-LRR基因作为一系列作物物种的半矮化基因，并应用Rht13在使用完美遗传标记的小麦育种计划中。