2-Hydroxychalcone as a Novel Natural Photosynthesis Inhibitor against Bloom-Forming Cyanobacteria,Journal of Agricultural and Food Chemistry

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2-Hydroxychalcone as a Novel Natural Photosynthesis Inhibitor against Bloom-Forming Cyanobacteria
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-11-29 , DOI: 10.1021/acs.jafc.2c06665
Xin Zhang ₁ , Xu Yang ₁ , Yichen Huang ₁ , Jinlu Hu ₂ , Diao Wu ₁ , Niu Yang ₁ , Haiying Wang ₁

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The control of harmful cyanobacterial blooms has been becoming a global challenge. The development of eco-friendly algicides with strong specificity is urgently needed. The photosynthetic apparatus is a promising target site for algicides to minimize the possible harmful effects on animals and humans. In this study, biologically derived 2-hydroxychalcone efficiently inhibited the growth of bloom-forming M. aeruginosa by selectively interfering with photosynthesis. 2-Hydroxychalcone targeting Photosystem II (PSII) inhibited electron transfer between the primary and secondary electron acceptors (Q_A and Q_B) and the binding of plastoquinone (PQ) molecules to the Q_B binding pocket at the acceptor side of PSII, as revealed by polyphasic chlorophyll (Chl) a fluorescence induction and Q_A^– reoxidation kinetics. Molecular docking for 2-hydroxychalcone to D1 protein and the proteomic responses of M. aeruginosa suggested that 2-hydroxychalcone formed a stable monodentate ligand with the nonheme iron in D1 protein, provoking significant modulation of PSII proteins. The unique binding mode of 2-hydroxychalcone with PSII differentiated it from classical PSII inhibitors. Furthermore, 2-hydroxychalcone down-regulated the expression of microcystin (MC) synthesis-related genes to restrain MC synthesis and release. These results indicated the potential application of 2-hydroxychalcone as an algicide or a template scaffold for designing novel derivatives with superior algicidal activity.

中文翻译：

2-羟基查耳酮作为一种新型天然光合作用抑制剂，可抑制蓝藻开花

有害蓝藻水华的控制已成为全球性挑战。迫切需要开发专一性强的环境友好型杀藻剂。光合装置是杀藻剂的一个有前途的目标场所，可以最大限度地减少对动物和人类可能产生的有害影响。在这项研究中，生物衍生的 2-羟基查耳酮通过选择性干扰光合作用，有效抑制了形成水华的铜绿假单胞菌的生长。靶向光系统 II (PSII) 的 2-羟基查尔酮抑制初级和次级电子受体（Q _A和 Q _B）之间的电子转移以及质体醌 (PQ) 分子与 Q _B的结合PSII 受体侧的结合口袋，如多相叶绿素 (Chl) 荧光诱导和 QA - 再氧化动力学所揭示_的^那样。2-羟基查耳酮与 D1 蛋白的分子对接和铜绿假单胞菌的蛋白质组学反应表明 2-羟基查耳酮与 D1 蛋白中的非血红素铁形成稳定的单齿配体，引起 PSII 蛋白的显着调节。2-羟基查耳酮与 PSII 的独特结合模式使其有别于经典的 PSII 抑制剂。此外，2-羟基查尔酮通过下调微囊藻毒素（MC）合成相关基因的表达来抑制MC的合成和释放。这些结果表明 2-羟基查尔酮作为杀藻剂或模板支架可用于设计具有优异杀藻活性的新型衍生物。

更新日期：2022-11-29

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