Rapid, simple, sensitive and reliable approaches for biogenic amines quantification in various food samples are essential to food safety. Lateral flow immunoassay (LFIA) has been wildly utilized in point-of-care testing (POCT) owing to its advantage of flexibility and feasibility. Here, we reported a Fe₃O₄@Au nanoparticles (NPs) (Fe₃O₄@AuNPs) based multimodal readout LFIA for rapid putrescine (Put) and histamine (His) quantification with a LOD down to 10 and 10 ng/mL in naked eye mode, 2.31 and 4.39 ng/mL in photothermal mode, 0.17 and 0.31 ng/mL in magnetic mode, respectively. Such multi-mode assay has been successfully used to detect Biogenic amines (BAs) in raw aquatic foods, including fish, prawns, beef, and pork, with overall recoveries ranging from 93.68 to 109.34%. Meanwhile, it is easily expanded to detect other typical BAs with high sensitivity by simply replacing antibodies. In view of the multi-signal reading, two quantitative formats, and high sensitivity, it may greatly widen the application of lateral flow detection in food safety.

对各种食品样品中的生物胺进行快速、简单、灵敏和可靠的定量方法对食品安全至关重要。侧向流免疫分析（LFIA）由于其灵活性和可行性的优势，已被广泛用于即时检测（POCT）。在这里，我们报道了 Fe ₃ O ₄ @Au 纳米粒子 (NPs) (Fe ₃ O ₄@AuNPs) 基于多模式读出 LFIA，用于快速定量腐胺 (Put) 和组胺 (His)，肉眼模式下 LOD 低至 10 和 10 ng/mL，光热模式下低至 2.31 和 4.39 ng/mL，0.17 和 0.31 ng/mL分别在磁模式下为 mL。这种多模式测定已成功用于检测生水产食品（包括鱼、虾、牛肉和猪肉）中的生物胺 (BA)，总回收率为 93.68% 至 109.34%。同时，通过简单地替换抗体，它很容易扩展到检测其他具有高灵敏度的典型 BA。鉴于多信号读数、两种定量格式和高灵敏度，它可能大大拓宽侧流检测在食品安全中的应用。