Approaches to DNA probe-mediated precision medicine have been extensively explored for the diagnosis and treatment of diverse types of cancer. Despite this, simple nanoscale devices with the required recognition specificity and sensitivity for clinical application have remained elusive until now. Here, we report a pH-driven covalent nanoscale device that integrates pH-responsive, switchable structure and proximity-driven covalent cross-linking. A tumor acidic, pH-driven mechanism eliminates “on-target, off-tumor” nonspecific recognition. By manipulating covalent binding to target molecule on the cell surface, this nanodevice avoids binding-then-shedding to improve the sensitivity of tumor recognition. We envision that this pH-driven covalent nanoscale device will inspire more clinical applications toward specific, long-term tumor imaging in the cancer microenvironment.

中文翻译：

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一种 pH 响应共价纳米器件，可增强特定肿瘤成像的时间和力稳定性

DNA 探针介导的精准医学方法已被广泛探索用于诊断和治疗各种类型的癌症。尽管如此，具有临床应用所需的识别特异性和敏感性的简单纳米级设备直到现在仍然难以捉摸。在这里，我们报告了一种 pH 驱动的共价纳米级装置，它集成了 pH 响应、可切换结构和接近驱动的共价交联。一种肿瘤酸性、pH 值驱动的机制消除了“靶向、脱瘤”的非特异性识别。通过操纵与细胞表面靶分子的共价结合，该纳米器件避免了先结合后脱落，从而提高了肿瘤识别的灵敏度。我们设想，这种 pH 驱动的共价纳米级设备将激发更多针对特定、