Oxidative stress induced by plasma-activated water stimulates astaxanthin production in Phaffia rhodozyma,Bioresource Technology

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Oxidative stress induced by plasma-activated water stimulates astaxanthin production in Phaffia rhodozyma
Bioresource Technology ( IF 9.7 ) Pub Date : 2022-11-21 , DOI: 10.1016/j.biortech.2022.128370
Wenshao Li ₁ , Carlos H Luna-Flores ₂ , Raveendra Anangi ₂ , Renwu Zhou ₃ , Xinle Tan ₄ , Marius Jessen ₂ , Lian Liu ₅ , Rusen Zhou ₆ , Tianqi Zhang ₇ , Alexander Gissibl ₂ , Patrick J Cullen ₇ , Kostya Ken Ostrikov ₁ , Robert E Speight ₈

Affiliation

School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.
School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia; School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia; State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, Shanxi 710049, People's Republic of China.
Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland (UQ), Brisbane, Queensland 4072, Australia.
Q-MAP, Metabolomics Australia, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland (UQ), Brisbane, Queensland 4000, Australia.
School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia; School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia.
School of Chemical and Biomolecular Engineering, The University of Sydney (USYD), Sydney, NSW 2006, Australia.
School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia; ARC Centre of Excellence in Synthetic Biology, Queensland University of Technology (QUT), Brisbane, Queensland 4000, Australia.

Astaxanthin is used extensively in the nutraceutical, aquaculture, and cosmetic industries. The current market necessitates higher astaxanthin production from Phaffia rhodozyma (P. rhodozyma) due to its higher cost compared to chemical synthesis. In this study, a bubble discharge reactor was developed to generate plasma-activated water (PAW) to produce PAW-made yeast malt (YM) medium. Due to oxidative stress induced by PAW, strains cultured in 15 and 30 min-treated PAW-made medium produced 7.9 ± 1.2 % and 12.6 ± 1.4 % more carotenoids with 15.5 ± 3.3 % and 22.1 ± 1.3 % more astaxanthin, respectively. Reactive oxygen species (ROS) assay results showed that ROS generated by plasma-water interactions elevated intracellular ROS levels. Proteomic analysis revealed increased expression of proteins involved in the cellular response to oxidative stress as well as carotenoid biosynthesis, both of which contribute to higher yields of astaxanthin. Overall, this study supports the potential of PAW to increase astaxanthin yields for industrial-scale production.

中文翻译：

等离子体活化水诱导的氧化应激刺激法夫酵母中虾青素的产生

虾青素广泛用于保健品、水产养殖和化妆品行业。目前的市场需要来自红发夫酵母( P. rhodozyma )的更高虾青素产量) 由于其成本高于化学合成。在这项研究中，开发了气泡放电反应器来生成等离子体活化水 (PAW)，以生产 PAW 制成的酵母麦芽 (YM) 培养基。由于 PAW 诱导的氧化应激，在经过 15 分钟和 30 分钟处理的 PAW 制成的培养基中培养的菌株分别产生了 7.9 ± 1.2 % 和 12.6 ± 1.4 % 的类胡萝卜素以及 15.5 ± 3.3 % 和 22.1 ± 1.3 % 的虾青素。活性氧 (ROS) 测定结果表明，血浆-水相互作用产生的 ROS 提高了细胞内 ROS 水平。蛋白质组学分析显示，参与细胞对氧化应激反应和类胡萝卜素生物合成的蛋白质表达增加，这两者都有助于提高虾青素的产量。全面的，

更新日期：2022-11-21

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