The main downside of utilizing algal biomass for biofuel production is the rigid cell wall which confines the availability of soluble organics to hydrolytic microbes during biofuel conversion. This constraint reduces the biofuel production efficiency of algal biomass. On the other hand, presenting various pretreatment methods before biofuel production affords cell wall disintegration and enhancement in biofuel generation. The potential of pretreatment methods chiefly relies on the extent of biomass liquefaction, energy, and cost demand. In this review, different pretreatments employed to disintegrate algal biomass were conferred in depth with detailed information on their efficiency in enhancing liquefaction and biofuel yield for pilot-scale implementation. Based on this review, it has been concluded that combinative and phase-separated pretreatments provide virtual input in enhancing the biofuel generation based on liquefaction potential, energy, and cost. Future studies should focus on decrement in cost and energy requirement of pretreatment in depth.

利用藻类生物质生产生物燃料的主要缺点是刚性细胞壁限制了可溶性有机物在生物燃料转化过程中水解微生物的可用性。这种限制降低了藻类生物质的生物燃料生产效率。另一方面，在生物燃料生产之前采用各种预处理方法可以分解细胞壁并增强生物燃料的产生。预处理方法的潜力主要取决于生物质液化程度、能源和成本需求。在这篇综述中，深入介绍了用于分解藻类生物质的不同预处理方法，并详细介绍了它们在提高液化和生物燃料产量方面的效率，以供中试规模实施。根据这篇评论，得出的结论是，组合预处理和分相预处理为基于液化潜力、能量和成本的生物燃料生产提供了虚拟输入。未来的研究应侧重于降低深度预处理的成本和能源需求。