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Enhancing ethylene glycol and ferric chloride pretreatment of rice straw by low-pressure carbon dioxide to improve enzymatic saccharification
Bioresource Technology ( IF 9.7 ) Pub Date : 2022-11-23 , DOI: 10.1016/j.biortech.2022.128391
Song Tang 1 , Yan-Ling Yu 2 , Rukuan Liu 3 , Shenghua Wei 4 , Qin Zhang 4 , Jie Zhao 4 , Song Li 4 , Qian Dong 5 , Yan-Bin Li 4 , Yuanli Wang 4
Affiliation  

Ethylene glycol and ferric chloride pretreatment assisted by low-pressure carbon dioxide (1 MPa CO2) realized the targeted deconstruction of lignocelluloses at 170 °C for 5 min, achieving 98 % cellulose recovery with removal of 92 % lignin and 90 % hemicellulose. After the pretreatment, the formation of stable platform mono-phenol components would be with the destruction of the lignin-carbohydrate complexes structure, and the surface of rice straw became rough, with a less negative charge and higher specific surface area, while the enzyme adsorption rate increased by 8.1 times. Furthermore, the glucose yield of pretreated straw was remarkably increased by 5.6 times that of the untreated straw, reaching 91 % after hydrolyzed for 48 h. With Tween 80 added in concentrated solid (12 %) hydrolysis at low cellulase loading (3 FPU/g dry substrate), half of the hydrolysis time was shortened than that without Tween 80, with 45 % higher glucose yield.



中文翻译:

低压二氧化碳强化乙二醇和氯化铁预处理水稻秸秆提高酶促糖化

低压二氧化碳(1 MPa CO 2 )辅助乙二醇和三氯化铁预处理) 实现了木质纤维素在 170 °C 下 5 分钟的靶向解构,实现了 98% 的纤维素回收,去除了 92% 的木质素和 90% 的半纤维素。预处理后,稳定的平台单酚组分的形成伴随着木质素-碳水化合物复合物结构的破坏,水稻秸秆表面变得粗糙,负电荷减少,比表面积增加,同时酶吸附率增长了8.1倍。此外,预处理秸秆的葡萄糖产率显着提高,是未处理秸秆的 5.6 倍,水解 48 小时后达到 91%。在低纤维素酶负载量(3 FPU/g 干底物)下,在浓缩固体 (12%) 水解中添加吐温 80 后,水解时间比未添加吐温 80 的水解时间缩短一半,葡萄糖产量提高 45%。

更新日期:2022-11-26
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