Bromophenols (BPs) have both natural and artificial sources in the environment and are frequently detected in plants. Herein, the ubiquitous 2,4,6-TriBP was hydroponically exposed to rice seedlings at two concentrations (0.2 and 2.0 mg/L) to characterize the dose-dependent abiotic stress responses of rice plants to BPs. The 2,4,6-TriBP induced oxidative damage to rice roots and subsequently inhibited plant transpiration and growth at the end of exposure in both concentrations. Moreover, the gene expression of OsUGT72B1 and the activity of glycosyltransferases of exposed rice roots were 2.36-to-4.41-fold and 1.23-to-1.72-fold higher than that of the blank controls after 24 h, following the formation of glycoconjugates in response to 2,4,6-TriBP exposure. It was notable that the glycosylation rates also showed a dose-effect relationship in rice roots. One and six glycoconjugates of 2,4,6-TriBP were detected in 0.2 and 2.0 mg/L exposure groups, respectively. Considering the detected species of glycoconjugates for four other types of BPs, the numbers of bromine atoms were found to dramatically affect their glycosylation process in rice plants. These results improve our fundamental understanding of the impact of congener structures and exposure concentrations of organic contaminants on the glycosylation process in response to phytotoxicity.

中文翻译：

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溴酚诱导水稻植物的多重胁迫反应：剂量和同类物结构的影响

溴酚 (BP) 在环境中既有天然来源，也有人工来源，并且经常在植物中检测到。在此，将无处不在的 2,4,6-TriBP 以两种浓度（0.2 和 2.0 mg/L）水培方式暴露于水稻幼苗，以表征水稻植物对 BPs 的剂量依赖性非生物胁迫反应。2,4,6-TriBP 对水稻根系造成氧化损伤，随后在两种浓度的暴露结束时抑制植物蒸腾和生长。此外，OsUGT72B1的基因表达24 小时后，暴露的水稻根的糖基转移酶活性分别比空白对照高 2.36 至 4.41 倍和 1.23 至 1.72 倍，随着糖缀合物的形成响应 2,4,6- TriBP 暴露。值得注意的是，糖基化率在水稻根系中也表现出剂量效应关系。在 0.2 和 2.0 mg/L 暴露组中分别检测到一种和六种 2,4,6-TriBP 糖缀合物。考虑到检测到的其他四种 BP 的糖缀合物种类，溴原子的数量被发现会显着影响它们在水稻植物中的糖基化过程。这些结果提高了我们对同类物结构和有机污染物暴露浓度对植物毒性反应糖基化过程影响的基本理解。