A simple fluorescent based organic fluorophore was synthesized and it shows significant fluorescent intensity with melatonin (MLN). Hence, it was applicable to the detection of MLN by colorimetric and fluorimetric techniques at neutral pH. Under optimized experimental condition, the synthesized organic fluorophore detects MLN selectively in the presence of other interfering biomolecules through ICT mechanism. The melatonin sensing mechanism is supported by DFT and ¹H-NMR titration. Based on the findings, this method can be applied to design a simple clinical diagnostic tool for MLN.

Graphical Abstract

中文翻译：

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基于安替比林的荧光团对生物体液中褪黑激素的高灵敏度和选择性检测

合成了一种简单的基于荧光的有机荧光团，它显示出与褪黑激素 (MLN) 显着的荧光强度。因此，它适用于在中性 pH 条件下通过比色法和荧光法检测 MLN。在优化的实验条件下，合成的有机荧光团通过 ICT 机制在存在其他干扰生物分子的情况下选择性地检测 MLN。褪黑激素传感机制得到 DFT 和¹ H-NMR 滴定的支持。基于这些发现，该方法可用于设计一种简单的 MLN 临床诊断工具。

图形概要