Tomato is one of the major vegetable crops grown worldwide and a model species for genetic and biological research. Progress in genomic technologies made possible the development of forefront methods for high-scale sequencing, providing comprehensive insight into the genetic architecture of germplasm resources. This review revisits next-generation sequencing strategies and applications to investigate the diversity of tomato, describing the common platforms used for SNP genotyping of large collections, de novo sequencing, and whole genome resequencing. Significant findings in evolutionary history are outlined, thus discussing how genomics has provided new hints about the processes behind domestication. Finally, achievement and perspectives on pan-genome construction and graphical pan-genome development toward precise mining of the natural variation to be exploited for breeding purposes are presented.

中文翻译：

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探索种质资源多样性的新一代测序技术：番茄的成就与趋势[J].

番茄是世界范围内种植的主要蔬菜作物之一，也是遗传和生物学研究的模式物种。基因组技术的进步使开发大规模测序的前沿方法成为可能，从而全面了解种质资源的遗传结构。这篇综述重新审视了下一代测序策略和应用，以研究番茄的多样性，描述了用于大型集合的 SNP 基因分型、从头测序和全基因组重测序的通用平台。概述了进化史上的重要发现，从而讨论了基因组学如何为驯化背后的过程提供新的线索。最后，