The β-lactoglobulin-chlorogenic acid (LG-CA) conjugate was explored to be formed through ultrasonication, redox-pair method and their combination, the ultrasonication used a probe ultrasonic machine with a 6 mm probe at 270 W, and the frequency was 20–25 kHz. The formation of the conjugate was confirmed by SDS-PAGE with a larger molecular weight. Besides, Fourier infrared spectroscopy (FTIR) and Circular dichroism (CD) indicated changes in the secondary structure of the LG-CA conjugate. The α-helix and β-sheet contents of LG decreased and the unordered content increased significantly after the formation of covalent complexes. In addition, both the ultrasonic treatment and its combination with redox-pair method could significantly improve the antioxidant properties of LG. The former increased to 23.16 μmol Trolox/g sample, the latter 82–106 μmol Trolox/g sample. Therefore, ultrasonication could be used both individually and in combination with the redox-pair method to produce LG-CA conjugates with stronger antioxidant activities.

β-乳球蛋白-绿原酸（LG-CA）结合物探索通过超声、氧化还原对法及其组合形成，超声采用6mm探头的探头超声机，功率270W，频率20 –25 赫兹。通过具有较大分子量的SDS-PAGE确认缀合物的形成。此外，傅立叶红外光谱 (FTIR) 和圆二色性 (CD) 表明 LG-CA 偶联物的二级结构发生了变化。共价复合物形成后LG的α-螺旋和β-折叠含量显着降低，无序含量显着增加。此外，超声波处理及其与氧化还原对法的结合都可以显着提高LG的抗氧化性能。前者增加到 23.16 μmol Trolox/g 样品，后者 82–106 μmol Trolox/g 样品。因此，超声处理可以单独使用，也可以与氧化还原对方法结合使用，以生产具有更强抗氧化活性的 LG-CA 结合物。